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  4. Human Folic acid(FA) ELISA Kit

Human Folic acid(FA) ELISA Kit

  • 中文名稱：
    人葉酸(FA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E17109h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人葉酸(FA)酶聯(lián)免疫試劑盒（CSB-E17109h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的FA含量。試劑盒檢測范圍為12.5 pg/mL-200 pg/mL，靈敏度為6.25 pg/mL。適用于營養(yǎng)學(xué)研究、代謝性疾病機(jī)制探索、藥物干預(yù)效果評估等科研領(lǐng)域，特別在葉酸代謝與妊娠健康、血液系統(tǒng)疾病關(guān)聯(lián)性研究中具有應(yīng)用價(jià)值。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N/A
  • 縮寫：
    FA
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    12.5 pg/mL-200 pg/mL
  • 靈敏度：
    6.25 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 89  
    Range % 84-93  
    1:40 Average % 93  
    Range % 89-97  
    1:80 Average % 95  
    Range % 90-100  
    1:160 Average % 84  
    Range % 80-88  
  • 回收率：
    The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-105  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.093 0.090 0.092    
    100 0.227 0.208 0.218    
    50 0.378 0.367 0.373    
    25 0.618 0.637 0.628    
    12.5 0.986 1.003 0.995    
    0 1.485 1.429 1.457    
  • 本試劑盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-human FA antibody.
      • Standard (10 x concentrate)(1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated FA (100 x concentrate) (1 x 6oμl)
      • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated FA antibody solution.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml)--Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

相關(guān)問答

■ 常見問題解答
Q:

We are looking for Elisa kit for human folic acid, detection range: 310-1000 nmol/L.
Material: erythrocytes (not full blood)
Please tell me is the result obtained from your kit can be comparable to the microbiological reference method?

A:
Thank you for your enquiry!Currently we just have this kit, and the detailed information, pls check the manual.
The theory of different detection methods are different.Since we have no information which is comparable to the microbiological reference method.
Pls just take your own actual test result.

靶點(diǎn)詳情

  • 最新研究進(jìn)展：
        葉酸（Folic acid）是一種維生素B族成員，參與人體DNA、RNA的合成和修復(fù)，對于細(xì)胞生長和分裂非常重要。最新的研究表明，葉酸在人體內(nèi)具有多種生理功能，包括對于心血管健康和神經(jīng)系統(tǒng)健康的影響，以及對于出生缺陷和某些類型的癌癥的預(yù)防作用。
        一項(xiàng)對臨床試驗(yàn)的回顧性分析發(fā)現(xiàn)，葉酸可以降低缺血性心血管疾?。ㄈ绻谛牟『湍X卒中）的發(fā)生率和死亡率。另一項(xiàng)研究顯示，葉酸可以促進(jìn)神經(jīng)細(xì)胞的發(fā)育和修復(fù)，有助于預(yù)防老年癡呆癥和帕金森病等神經(jīng)系統(tǒng)疾病的發(fā)生。
    此外，葉酸還可以預(yù)防某些類型的出生缺陷，如脊柱裂和唇裂等，因?yàn)檫@些缺陷通常與母體葉酸不足有關(guān)。對于某些類型的癌癥，如結(jié)腸癌和乳腺癌等，一些研究表明，適當(dāng)?shù)娜~酸攝入可以減少其發(fā)生的風(fēng)險(xiǎn)。