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  4. Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

Rat Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

  • 中文名稱：
    大鼠粒細(xì)胞集落刺激因子(G-CSF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07340r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠粒細(xì)胞集落刺激因子(G-CSF)酶聯(lián)免疫試劑盒（CSB-E07340r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的G-CSF含量。G-CSF即粒細(xì)胞集落刺激因子，在造血調(diào)控中至關(guān)重要。它能特異性地作用于造血干細(xì)胞，促進(jìn)粒細(xì)胞前體細(xì)胞增殖、分化，增強成熟粒細(xì)胞功能。臨床常用于放化療后骨髓抑制等，提升粒細(xì)胞數(shù)量，降低感染風(fēng)險，研究聚焦其信號通路及新應(yīng)用。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，在基礎(chǔ)研究中可用于探索免疫系統(tǒng)調(diào)控機制、炎癥相關(guān)疾病模型中的G-CSF動態(tài)變化，或評估藥物/化合物對中性粒細(xì)胞生成的影響。例如在感染性疾病模型、骨髓抑制恢復(fù)研究或組織損傷修復(fù)實驗中，可通過檢測G-CSF表達(dá)水平揭示其生物學(xué)功能。試劑盒操作流程標(biāo)準(zhǔn)化，配備預(yù)包被96孔板及優(yōu)化反應(yīng)體系，適用于實驗室常規(guī)批量樣本檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
  • 縮寫：
    G-CSF
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.81 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat G-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-94
    1:2Average %101
    Range %97-104
    1:4Average %95
    Range %90-97
    1:8Average %100
    Range %95-103
  • 回收率：
    The recovery of rat G-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9492-96
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.133 2.118 2.126 2.007
    10001.837 1.776 1.807 1.688
    5001.294 1.282 1.288 1.169
    2500.889 0.878 0.884 0.765
    1250.588 0.607 0.598 0.479
    62.50.361 0.349 0.355 0.236
    31.250.222 0.216 0.219 0.100
    00.118 0.120 0.119 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 基因功能參考文獻(xiàn)：
    1. Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID: 28550897
    2. Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID: 27364613
    3. The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID: 25677907
    4. Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID: 12071442
    5. GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID: 14557677
    6. We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID: 14651956
    7. GM-CSF may play a significant role in follicular development PMID: 15958400
    8. GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID: 16276414
    9. GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID: 16877635
    10. GM-CSF acts as a neuroprotective protein in the CNS PMID: 17457367
    11. Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID: 17478029
    12. GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID: 17953750
    13. G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID: 18832728
    14. IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID: 19022682

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    GM-CSF family
  • 數(shù)據(jù)庫鏈接：