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  4. Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

  • 中文名稱：
    大鼠粒細胞巨噬細胞集落刺激因子(GM-CSF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E04570r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠粒細胞巨噬細胞集落刺激因子(GM-CSF)酶聯(lián)免疫試劑盒（CSB-E04570r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CSF2含量。CSF2，即粒細胞-巨噬細胞集落刺激因子（GM-CSF），是一種由多種細胞分泌的細胞因子，最初被定義為一種造血因子。它通過調(diào)節(jié)骨髓細胞生成和免疫反應(yīng)，在免疫調(diào)節(jié)、炎癥反應(yīng)和腫瘤進展中扮演關(guān)鍵角色。CSF2通過激活信號通路，如JAK-STAT和PI3K-Akt，影響細胞的增殖、分化和功能，并在多種疾病的治療中具有潛在應(yīng)用價值。試劑盒檢測范圍為15/6 pg/ml- 1000 pg/ml，靈敏度為3.9 pg/ml。適用于科研領(lǐng)域中對大鼠感染性疾病模型、免疫調(diào)控機制、造血功能研究以及藥物干預(yù)效果的評價。實驗操作兼容多種生物樣本類型，可滿足體外實驗中GM - CSF的動態(tài)監(jiān)測需求，為探索免疫相關(guān)疾病的分子機制或評估實驗處理對細胞因子表達的影響提供可靠工具，廣泛應(yīng)用于基礎(chǔ)醫(yī)學、藥理學及免疫學等研究場景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/ml - 1000 pg/ml
  • 靈敏度：
    3.9 pg/ml
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    85

    Range %

    81-89

    1:2

    Average %

    97

    Range %

    92-101

    1:4

    Average %

    91

    Range %

    86-95

    1:8

    Average %

    103

    Range %

    99-107

  • 回收率：

    The recovery of rat GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    88

    84-92

    EDTA plasma (n=4)

    94

    90-98

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.468

    2.326

    2.397

    2.210

    500

    1.857

    1.792

    1.825

    1.638

    250

    1.213

    1.250

    1.232

    1.045

    125

    0.785

    0.813

    0.799

    0.612

    62.5

    0.492

    0.468

    0.480

    0.293

    31.2

    0.331

    0.347

    0.339

    0.152

    15.6

    0.289

    0.271

    0.280

    0.093

    0

    0.184

    0.189

    0.187

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 基因功能參考文獻：
    1. Transplant vasculopathy depends on the simultaneous and complementary additive effects of granulocyte-macrophage colony-stimulating factor and cytokines, which have a continuous "cross talk." PMID: 28550897
    2. Mesenteric mesothelial cells express GM-CSF receptor on the plasma membrane; during inflammation they synthesize and secrete GM-CSF that can stimulate GM-CSF receptor expression and mesothelial cell to macrophage-like cell transformation; in this transformation, GM-CSF (together with its receptor and other cytokines) has a major autocrine regulatory role; receptor-ligand internalization is essential for GM-CSF signalling PMID: 27364613
    3. The findings that ephrinA1-PE38/GM-CSF had a high efficacy in the activation of the dendritic cells would facilitate the development of in vivo dendritic-cell vaccines for the treatment of gliomas in rats PMID: 25677907
    4. Monocyte chemotactic protein-1 and GM-CSF may play an important role in the progression of experimental autoimmune myocarditis PMID: 12071442
    5. GM-CSF exerts a neuromodulatory effect and may provide a new link of neuroimmune communication. PMID: 14557677
    6. We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. PMID: 14651956
    7. GM-CSF may play a significant role in follicular development PMID: 15958400
    8. GM-CSF signaling in the central nervous system can regulate energy homeostasis. PMID: 16276414
    9. GM-CSF signaling as reflected by PU.1 expression and nuclear binding was restored with recombinant GM-CSF treatment in vitro. PMID: 16877635
    10. GM-CSF acts as a neuroprotective protein in the CNS PMID: 17457367
    11. Maternal administration of GM-CSF induced fetal lung maturation and this effect may be mediated, at least partly, by an increase in the eNOS expression. PMID: 17478029
    12. GM-CSF stimulates neuronal differentiation of adult neural stem cells. PMID: 17953750
    13. G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. PMID: 18832728
    14. IL-1beta promotes tissue catabolism and destruction, whereas GM-CSF enhances tissue reconstruction PMID: 19022682

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    GM-CSF family
  • 數(shù)據(jù)庫鏈接：