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  4. Rat Interleukin 5,IL-5 ELISA KIT

Rat Interleukin 5,IL-5 ELISA KIT

  • 中文名稱：
    大鼠白介素5(IL-5)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07435r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素5(IL-5)酶聯(lián)免疫試劑盒（CSB-E07435r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IL5含量。IL-5 是一種重要的細(xì)胞因子，在嗜酸性粒細(xì)胞的生長、分化及存活中發(fā)揮關(guān)鍵作用。其過度表達(dá)與多種炎癥性疾病如哮喘、嗜酸性粒細(xì)胞增多癥等相關(guān)。研究聚焦于阻斷 IL-5 信號(hào)通路，以減少嗜酸性粒細(xì)胞數(shù)量，從而緩解相關(guān)疾病的炎癥癥狀。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎(chǔ)科研領(lǐng)域中IL-5相關(guān)免疫調(diào)節(jié)機(jī)制研究，如構(gòu)建哮喘動(dòng)物模型時(shí)評(píng)估炎癥因子水平，或體外藥物篩選實(shí)驗(yàn)中監(jiān)測細(xì)胞因子分泌動(dòng)態(tài)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %82-91
    1:2Average %92
    Range %83-99
    1:4Average %101
    Range %93-107
    1:8Average %96
    Range %90-104
  • 回收率：
    The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8885-94
    EDTA plasma (n=4)9490-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.407 2.457 2.432 2.268
    5001.768 1.736 1.752 1.588
    2501.065 1.098 1.082 0.918
    1250.704 0.735 0.720 0.556
    62.50.448 0.469 0.459 0.295
    31.20.323 0.300 0.312 0.148
    15.60.221 0.229 0.225 0.061
    00.161 0.167 0.164 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-5 family
  • 數(shù)據(jù)庫鏈接：