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  4. Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

Rat N-terminal procollagen Ⅲ propeptide,PⅢNP ELISA Kit

  • 中文名稱：
    大鼠Ⅲ型前膠原氨基末端肽(PⅢNP)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E08096r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠Ⅲ型前膠原氨基末端肽(PⅢNP)酶聯(lián)免疫試劑盒（CSB-E08096r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PⅢNP含量。PⅢNP（Ⅲ型前膠原氨基端肽）是一種與組織纖維化相關(guān)的生物標(biāo)志物。它在多種疾病如慢性心力衰竭和腦積水的發(fā)展過程中扮演重要角色。研究顯示，PⅢNP水平與疾病嚴(yán)重程度相關(guān)，可作為評估預(yù)后的指標(biāo)。其具體作用機制涉及細胞外基質(zhì)重塑和纖維化過程。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，適用于科研領(lǐng)域?qū)δz原代謝相關(guān)機制的研究，適用于動物模型中的纖維化疾病機制探索、抗纖維化藥物研發(fā)效果評估，以及組織工程研究中膠原代謝動態(tài)監(jiān)測。通過檢測不同樣本類型中的PⅢNP水平，研究者可深入探究膠原沉積與降解平衡在疾病發(fā)生發(fā)展中的作用，為相關(guān)科研項目提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    N-terminal procollagen 3 propeptide,P3NP,_x000D_ N-terminal procollagen III propeptide,PIIINP
  • 縮寫：
    PⅢNP
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.625 ng/mL-40 ng/mL
  • 靈敏度：
    0.156 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅢNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-101  
    1:2 Average % 98  
    Range % 93-102  
    1:4 Average % 92  
    Range % 87-95  
    1:8 Average % 95  
    Range % 91-98  
  • 回收率：
    The recovery of rat PⅢNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 85-94  
    EDTA plasma (n=4) 98 94-105  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.102 2.077 2.090 1.959  
    20 1.412 1.447 1.430 1.299  
    10 0.924 0.945 0.935 0.804  
    5 0.592 0.608 0.600 0.469  
    2.5 0.360 0.374 0.367 0.236  
    1.25 0.289 0.271 0.280 0.149  
    0.625 0.211 0.222 0.217 0.086  
    0 0.130 0.132 0.131    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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