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  4. Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

  • 中文名稱：
    大鼠NAD依賴性蛋白脫乙酰酶sirtuin-1(SIRT1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL021339RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠NAD依賴性蛋白脫乙酰酶sirtuin-1(SIRT1)酶聯(lián)免疫試劑盒（CSB-EL021339RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的SIRT1含量。SIRT1 是一種具有重要生物學(xué)意義的靶點(diǎn)。它參與細(xì)胞代謝、衰老、應(yīng)激反應(yīng)等多種生理過(guò)程。研究發(fā)現(xiàn)它通過(guò)去乙?；饔谜{(diào)控下游蛋白功能，在癌癥、代謝疾病等方面有潛在機(jī)制，是當(dāng)前藥物研發(fā)和基礎(chǔ)研究關(guān)注的重要靶點(diǎn)。試劑盒檢測(cè)范圍為34.3 pg/mL-2200 pg/mL，適用于體外研究，如通過(guò)檢測(cè)不同處理?xiàng)l件下（如藥物干預(yù)、基因修飾）的SIRT1表達(dá)變化，探索其在能量穩(wěn)態(tài)調(diào)節(jié)、神經(jīng)保護(hù)或抗氧化通路中的作用機(jī)制，也可用于肥胖、糖尿病等代謝模型動(dòng)物的生物標(biāo)志物分析。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
  • 縮寫(xiě)：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    34.3 pg/mL-2200 pg/mL
  • 靈敏度：
    8.5 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %86-94
    1:2Average %102
    Range %97-105
    1:4Average %91
    Range %87-95
    1:8Average %98
    Range %92-102
  • 回收率：
    The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9995-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    22002.357 2.272 2.315 2.209
    11001.976 1.873 1.925 1.819
    5501.582 1.486 1.534 1.428
    2751.141 1.158 1.150 1.044
    137.50.761 0.757 0.759 0.653
    68.750.436 0.425 0.431 0.325
    34.30.262 0.266 0.264 0.158
    00.107 0.105 0.106
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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