搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rat Nephrin ELISA Kit

Rat Nephrin ELISA Kit

  • 中文名稱：
    大鼠腎病蛋白(nephrin)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13957r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腎病蛋白(nephrin)酶聯(lián)免疫試劑盒（CSB-E13957r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NPHS1含量。NPHS1 是一個重要靶點，它與腎臟疾病緊密相關，其編碼的蛋白參與腎小球濾過屏障的構成。研究發(fā)現(xiàn)，NPHS1 突變可導致先天性腎病綜合征。學者們正深入研究其作用機制，期望為相關腎病的診斷和治療帶來新策略。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL，適用于科研領域中對腎小球足細胞相關功能的研究；適用于體外研究腎臟疾病模型中足細胞損傷機制、藥物對腎小球結(jié)構影響的評估，或通過檢測組織勻漿探索腎小球發(fā)育及病理改變相關的分子機制本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Nphs1 ELISA Kit; Nphn ELISA Kit; Nephrin ELISA Kit; Renal glomerulus-specific cell adhesion receptor ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-80 ng/mL
  • 靈敏度：
    0.312 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Nephrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-95
    1:2Average %95
    Range %91-98
    1:4Average %95
    Range %81-89
    1:8Average %100
    Range %97-103
  • 回收率：
    The recovery of rat Nephrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10197-105
    EDTA plasma (n=4)9895-102
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.215 2.194 2.205 2.049
    401.846 1.804 1.825 1.669
    201.444 1.468 1.456 1.300
    101.033 1.068 1.051 0.895
    50.634 0.671 0.653 0.497
    2.50.373 0.398 0.386 0.230
    1.250.297 0.298 0.298 0.142
    00.154 0.157 0.156
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.
  • 基因功能參考文獻：
    1. Data show that metformin provides some protection against podocyte damage in diabetic rats, which may be related, in part, to its effect in reducing urinary nephrin (Nphs1) excretion as well as improving podocyte nephrin expression. PMID: 27248136
    2. The effects of sildenafil citrate on urinary podocin and nephrin mRNA expression in an L-NAME model of pre-eclampsia. PMID: 27995418
    3. Irbesartan treatment ameliorates proteinuria in puromycin aminonucleoside nephropathy by inhibiting the reduction of NEPH1 and nephrin in the kidney glomerulus. PMID: 25298195
    4. These findings suggest that candesartan can prevent cisplatin-induced testicular damage by regulating expression patterns of the nephrin-podocin complex in the testes. PMID: 26539476
    5. Rat nephrin Tyr1127 and Tyr1152 are essential for SHP-1 induced nephrin dephosphorylation under high-glucose conditions. PMID: 25404734
    6. Resveratrol alleviated dyslipidemia and renal damage induced by high-fat diet also reduced TNF-alpha and MCP-1 levels, and reduced expressions of nephrin and podocin. PMID: 25025298
    7. findings show that the filament-forming GTPase septin 7 is expressed in podocytes and associates with CD2-associated protein (CD2AP) and nephrin, both essential for glomerular ultrafiltration PMID: 22809625
    8. Soy protein preserves basement membrane integrity through a synergistic effect on nephrin, matrix metalloproteinase and vascular endothelial growth factor PMID: 22094487
    9. Huaiqihuang Granule can up-regulate the expressions of nephrin and podocin in renal tissues of rats with adriamycin-induced nephrosis. PMID: 21565142
    10. The decrease of nephrin expression and the change of its distribution might be the significant factors resulting in considerable proteinuria. PMID: 20629321
    11. Abnormal expression of nephrin is the important molecular mechanism that leads to the occurrence and development of proteinuria. PMID: 20045917
    12. These observations indicate that podocyte-specific expression of rat nephrin can rescue nephrin-deficient mice from perinatal death, but is not sufficient for full complementation. PMID: 19948823
    13. nephrin anchors the slit diaphragm to the actin cytoskeleton, possibly by linkage to CD2AP PMID: 11880318
    14. reduction in nephrin may be a determinant of glomerular hyperpermeability in diabetic nephropathy PMID: 12105259
    15. in normotensive and hypertensive rats, nephrin expression increased from birth to the adult age and down-regulation of nephrin in SHR evident from the early developmental kidney to adulthood may contribute to the development of albuminuria in adult SHR. PMID: 12109777
    16. Expression of nephrin in proteinuric states and in developing glomeruli. Review. PMID: 12386277
    17. Depletion in glomerular nephrin expression in diabetic animals was only abrogated by valsartan treatment, the therapy which was most effective at retarding the development of albuminuria in this model. PMID: 12544453
    18. Nephrin and Neph1 form a hetero-oligomeric receptor complex in the plane of the membrane that might interact across the foot process intercellular junction through interactions between Nephrin with itself and Neph1 PMID: 12646566
    19. nephrin and Neph1 interact and have roles in glomerular permeability PMID: 12865409
    20. Onset of proteinuria in passive Heymann nephritis model of membranous nephropathy is coincident with complement-dependent alterations in association of nephrin with actin cytoskeleton and loss of podocyte slit diaphragm integrity. PMID: 14633129
    21. Nephrin expression and its distribution were altered in puromycin aminonucleoside nephrosis in rats. PMID: 15385636
    22. Data show that in glomerulonephritis in rats, an upregulation of nephrin expression occurs with a concomitant transient downregulation of podocin and CD2AP which may account for a highly dysregulated filtration barrier and increased proteinuria. PMID: 15465010
    23. NPHN contributes to a porous slit diaphragm scaffold, the molecular filter in renal glomerular capillaries. PMID: 15545998
    24. Fetal membranes and placenta of the rat express mRNA for the protein barriers nephrin and Neph 1, suggesting a role in the regulation of protein transfer from the fetus to mother PMID: 15979540
    25. Nck couples nephrin to the actin cytoskeleton in glomerular podocytes and contributes to the maintenance of normal morphology and function of podocytes PMID: 16934223
    26. Downregulation of glomerular Nephrin gene expression in minimal-change like nephropathy. PMID: 17429054
    27. Darbepoetin treatment ameliorates podocyte injury and decreases proteinuria by a direct effect on podocytes. This may be accomplished by maintenance of the actin cytoskeleton and nephrin expression. PMID: 17457371
    28. the nephrin gene is differentially expressed according to glomerular size PMID: 17599973
    29. PI3K is involved in nephrin-mediated actin reorganization in podocytes. PMID: 18033240
    30. Tyrosine phosphorylation of nephrin by regulating F-actin formation may be important for the maintenance of normal podocyte morphology and function. PMID: 18256598
    31. Neph1 but not nephrin specifically binds to adaptor protein Grb2 and tyrosine kinase Csk in a phosphorylation-dependent manner. PMID: 18258597
    32. PEDF could reduce proteinuria by suppressing podocyte damage and decreased nephrin as well as increased VEGF expression in the glomeruli of nephrotic syndrome model rats. PMID: 19042927
    33. Nck is required to maintain adult podocytes and phosphotyrosine-based interactions with nephrin may occur in foot processes of resting, mature podocytes. PMID: 19443634

    顯示更多

    收起更多

  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Immunoglobulin superfamily
  • 組織特異性：
    Strongly expressed in the podocytes of kidney glomeruli (at protein level) and at lower levels in the spleen.
  • 數(shù)據(jù)庫鏈接：