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  4. Rat Neprilysin(MME) ELISA kit

Rat Neprilysin(MME) ELISA kit

  • 中文名稱：
    大鼠腦啡肽酶(MME)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL014653RA
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠腦啡肽酶(MME)酶聯(lián)免疫試劑盒（CSB-EL014653RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MME含量。MME即膜金屬肽酶，它在人體生理過程如血壓調(diào)節(jié)、神經(jīng)信號傳導(dǎo)等方面有重要作用。研究中其機制主要聚焦于作為藥物靶點，通過調(diào)節(jié)其活性來干預(yù)特定疾病進展，如在心血管疾病、神經(jīng)系統(tǒng)疾病等領(lǐng)域是潛在治療方向。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，適用于基礎(chǔ)科研中探究神經(jīng)系統(tǒng)功能、藥物干預(yù)對MME活性調(diào)控、疾病模型機制分析等方向，為神經(jīng)生物學(xué)、藥理學(xué)及病理學(xué)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    MmeNeprilysin ELISA Kit; EC 3.4.24.11 ELISA Kit; Atriopeptidase ELISA Kit; Enkephalinase ELISA Kit; Neutral endopeptidase 24.11 ELISA Kit; NEP ELISA Kit; Neutral endopeptidase ELISA Kit; Skin fibroblast elastase ELISA Kit; SFE ELISA Kit; CD antigen CD10 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    47 pg/mL-3000 pg/mL
  • 靈敏度：
    11.75 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MME in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 92
    Range % 89-96
    1:2 Average % 96
    Range % 91-103
    1:4 Average % 98
    Range % 93-101
    1:8 Average % 95
    Range % 90-99
  • 回收率：
    The recovery of rat MME spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 89-96
    EDTA plasma (n=4) 98 93-103
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    3000 2.256 2.345 2.301 2.104
    1500 1.634 1.536 1.585 1.388
    750 1.128 1.214 1.171 0.974
    375 0.774 0.787 0.781 0.584
    187.5 0.479 0.482 0.481 0.284
    94 0.357 0.365 0.361 0.164
    47 0.268 0.269 0.269 0.072
    0 0.195 0.199 0.197 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of the atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • 基因功能參考文獻：
    1. hypoxia increased the expression of platelet-derived growth factor (PDGF) and transforming growth factor-beta1 (TGF-beta1) and decreased the expression of neprilysin (NEP), which contributed to the hypoxia-induced Endothelial-to-mesenchymal transition of pulmonary artery endothelial cells. PMID: 27373199
    2. The data obtained suggest that the increase of caspase-3 enzyme activity could affect NEP expression via proteolytic degradation of its transcription factor AICD PMID: 26983277
    3. the increased expression of NPR-C (r=-0.943 and -0.837 for mRNA and protein respectively, p<0.05) and NEP (r=-0.687 and -0.823 for mRNA and protein respectively, p<0.05) were accompanied by a signi fi cant decline in CNP. PMID: 24189506
    4. Data suggest that the brain expression of neprilysin may promote amyloid beta-protein (Abeta)deposition in patients with Alzheimer's disease (AD). PMID: 24508800
    5. These results suggested that ketamine suppresses the Abeta degradation of NEP by reducing p38 MAPK-mediated pathway activity. PMID: 23624023
    6. Naoerkang can increase the expression of NEP in hippocampal tissues of AD rats so as to reduce the quantity of AAbeta(1-42). PMID: 20141738
    7. The three Abeta-degrading enzymes were damaged to different extents in the brain of diabetic rats, and impairment of ECE-1 and IDE partly contributed to the elevated Abeta(1-40) levels in brain of diabetic rats. PMID: 20414044
    8. Androgen regulation of amyloid beta (Abeta)protein involves an androgen receptor-dependent mechanism requiring up-regulation of Abeta catabolizing enzyme neprilysin. PMID: 18346198
    9. Ovariectomy increases BACE1 expression and decreases neprilysin expression in hippocampus. PMID: 20137687
    10. Intracardiac expression of neutral endopeptidase PMID: 12030369
    11. in spontaneously hypertensive rats, the local concentration and action of adrenomedullin in the tissues may be differentially regulated by NEP. PMID: 15005274
    12. Neutral endopeptidase inactivity induces an exaggerated neurogenic inflammation mediated by neurokinin-1 receptor, which may lead to edema in pulmonary alveoli. PMID: 15194566
    13. Report that CGS 35601 and its orally active prodrug CGS 37808 are triple inhibitors of endothelin-converting enzyme-1, neutral endopeptidase 24.11, and angiotensin-converting enzyme. PMID: 15838282
    14. Examine the effect of triple vasopeptidase inhibition of angiotensin-converting enzyme/neutral endopeptidase/endothelin-converting enzyme activities on the hemodynamic profile of spontaneously hypertensive rats. PMID: 15838331
    15. neutral endopeptidase activity is decreased by ascorbic acid in cultured osteoblastic cells PMID: 15908023
    16. The promoter of NEP is frequently methylated in hepatocellular carcinoma in contrast to its unmethylated status in normal liver. PMID: 16827801
    17. These results demonstrate that expression of neprilysin and aminopeptidase N can be regulated when peptide neurons are activated and, that regulation is enzyme-, region-, and stage-specific. PMID: 17952634
    18. NEP inhibition potentiates substance P-mediated neutrophil oxygen radical production and may promote other inflammatory activities during Mg-deficiency PMID: 18607539
    19. chromatin immunoprecipitation analysis also reveals amyloid precursor protein binding to the NEP promoter in rat primary neurons but not in HUVEC cells PMID: 19057576
    20. results show that acute immobilization stress affects differentially enkephalinase and oxytocinase activities depending on the fraction and brain region analyzed PMID: 19468242

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  • 亞細胞定位：
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Peptidase M13 family
  • 數(shù)據(jù)庫鏈接：