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  4. Rat Pancreatic prohormone(PPY) ELISA kit

Rat Pancreatic prohormone(PPY) ELISA kit

  • 中文名稱：
    大鼠胰多肽酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E12747r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠胰多肽酶聯(lián)免疫試劑盒（CSB-E12747r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的PPY含量。PPY即多肽YY，是由腸道L細(xì)胞分泌的一種激素。它能調(diào)節(jié)食欲、能量代謝等。研究機(jī)制上，其可與特定受體結(jié)合，作用于下丘腦等腦區(qū)以抑制食欲；也能影響胃腸道運(yùn)動(dòng)和消化液分泌，在維持機(jī)體能量穩(wěn)態(tài)等生理過(guò)程中發(fā)揮重要作用。試劑盒檢測(cè)范圍為0.78 pg/mL-50 pg/mL，適用于科研場(chǎng)景中探究胰多肽在消化系統(tǒng)疾病模型、代謝調(diào)控機(jī)制或藥物干預(yù)實(shí)驗(yàn)中的動(dòng)態(tài)變化，為動(dòng)物實(shí)驗(yàn)提供可靠的內(nèi)分泌指標(biāo)檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 縮寫：
    PPY
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    0.78 pg/mL-50 pg/mL
  • 靈敏度：
    0.2 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 104    
    Range % 93-109    
    1:200 Average % 100    
    Range % 91-104    
    1:400 Average % 97    
    Range % 88-101    
    1:800 Average % 90    
    Range % 82-94    
  • 回收率：
    The recovery of rat PPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 83-94    
    EDTA plasma (n=4) 100 91-104    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    50 2.069 1.932 2.001 1.899    
    25 1.429 1.466 1.448 1.346    
    12.5 0.946 0.917 0.932 0.830    
    6.25 0.662 0.651 0.657 0.555    
    3.12 0.348 0.340 0.344 0.242    
    1.56 0.242 0.237 0.240 0.138    
    0.78 0.164 0.159 0.162 0.060    
    0 0.104 0.100 0.102 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    NPY family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: rno:24677

    STRING: 10116.ENSRNOP00000028318

    UniGene: Rn.9755