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  4. Rat Tissue factor,TF ELISA Kit

Rat Tissue factor,TF ELISA Kit

  • 中文名稱：
    大鼠組織因子(TF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E07914r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠組織因子(TF)酶聯(lián)免疫試劑盒（CSB-E07914r）為雙抗夾心法ELISA試劑盒，定量檢測血清、尿液、組織培養(yǎng)上清液、組織勻漿、細胞裂解物樣本中的F3含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，試劑盒適用于心血管疾病模型、腫瘤生物學研究、炎癥相關(guān)實驗等科研場景，通過檢測不同樣本類型中TF的動態(tài)變化，可深入探究其在疾病發(fā)生發(fā)展中的作用機制，或用于藥物干預(yù)實驗中生物標志物的效果評估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, urine, cell culture supernates, tissue homogenates, cell lysates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Blood Coagulation
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %102
    Range %91-106
    1:2Average %90
    Range %85-96
    1:4Average %93
    Range %89-100
    1:8Average %99
    Range %93-108
  • 回收率：
    The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8884-94
    EDTA plasma (n=4)10395-107
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.297 2.231 2.264 2.081
    10001.573 1.471 1.522 1.339
    5000.999 0.924 0.962 0.779
    2500.618 0.593 0.606 0.423
    1250.405 0.425 0.415 0.232
    62.50.293 0.310 0.302 0.119
    31.250.249 0.264 0.257 0.074
    00.177 0.188 0.183 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 亞細胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Tissue factor family
  • 數(shù)據(jù)庫鏈接：