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  • 中文名稱:
    大鼠三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E05085r
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒(CSB-E05085r)為競(jìng)爭(zhēng)法ELISA試劑盒,定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的T3含量。靶點(diǎn)研究旨在識(shí)別疾病相關(guān)分子,作為藥物干預(yù)目標(biāo)。通過(guò)生物信息學(xué)、基因組學(xué)等技術(shù),挖掘差異表達(dá)基因,構(gòu)建蛋白質(zhì)相互作用網(wǎng)絡(luò),尋找關(guān)鍵節(jié)點(diǎn)和基因簇,預(yù)測(cè)潛在治療藥物,為疾病治療提供新思路。試劑盒檢測(cè)范圍為0.5 ng/mL-8 ng/mL,靈敏度為0.5 ng/mL。適用于科研領(lǐng)域中對(duì)甲狀腺激素代謝機(jī)制、藥物干預(yù)效果評(píng)估、疾病動(dòng)物模型構(gòu)建等研究需求,為體外實(shí)驗(yàn)提供穩(wěn)定可靠的檢測(cè)工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě):
    T3
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍:
    0.5 ng/mL-8 ng/mL
  • 靈敏度:
    0.5 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

     

    Three samples of known concentration were tested twenty times on one plate to assess.

     

    Inter-assay Precision (Precision between assays): CV%<15%

     

    Three samples of known concentration were tested in twenty assays to assess.

      

  • 標(biāo)準(zhǔn)曲線:

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本試劑盒所含材料:
      • A 96-well Assay plate --The 96-well plate has been pre-coated with an anti-human Ts antibody.
      • Standard(10 x concentrate) (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Biotin-conjugated T3 (1 x  6 ml)
      • HRP-avidin (1 x 6 ml) --Catalyze the TMB, eliciting a chromogenic reaction.
      • Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
      • Substrate A (1x 7 ml) --Mix with Substrate B.
      • Substrate B (1x 7 ml) --Mix with Substrate A.
      • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本試劑盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 2 個(gè)評(píng)價(jià)

樣品類型:血清

樣品信息:大鼠

稀釋比:其他 1:10

產(chǎn)品評(píng)價(jià): 我用CSB-E05085r檢測(cè)自身免疫性甲狀腺炎大鼠,稀釋比例為1:10。測(cè)試結(jié)果符合內(nèi)分泌軸甲狀腺負(fù)反饋,趨勢(shì)準(zhǔn)確,支持國(guó)貨,性價(jià)比無(wú)敵,國(guó)產(chǎn)最好用的

By 丁老師

樣品類型:血清

樣品信息:大鼠

稀釋比:沒(méi)有稀釋

產(chǎn)品評(píng)價(jià): 我用CSB-E05085r ELISA試劑盒 檢測(cè)甲亢大鼠血清樣本,其OD值為0.045,標(biāo)準(zhǔn)曲線滿足要求,檢測(cè)樣本數(shù)據(jù)可靠,符合實(shí)驗(yàn)預(yù)期,順利完成實(shí)驗(yàn)。試劑盒操作簡(jiǎn)單方便,非常好用

By 邱老師

相關(guān)問(wèn)答

 常見(jiàn)問(wèn)題解答
Q:

Please let us know delivery time and the sulfuric acid content of [stop solution] .

A:
Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.
Q:

1) Does this kit contain any preservatives?
3) Could you let me know expression system of the standard recombinants in this kit?

A:
Thanks for your inquiry. Preservative: proclin 300(dilution ratio: 1:3000);
Standards: small molecule.

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    最新研究表明,T3對(duì)機(jī)體的作用不僅限于甲狀腺功能和代謝調(diào)節(jié),它還能影響多種細(xì)胞類型的功能和信號(hào)傳遞,如心肌細(xì)胞、神經(jīng)元和免疫細(xì)胞等。這些發(fā)現(xiàn)揭示了T3在整個(gè)生命體系中的重要作用。