Sheep tri-iodothyronine (T3) ELISA kit

文獻(xiàn)引用 (1) 說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

綿羊三碘甲狀腺原氨酸(T3)酶聯(lián)免疫試劑盒（CSB-EQ027510SH）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的T3含量。試劑盒檢測(cè)范圍為0.5 ng/mL-8 ng/mL，靈敏度為0.5 ng/mL。適用于科研領(lǐng)域中對(duì)甲狀腺激素代謝調(diào)控機(jī)制的研究；適用于動(dòng)物生理學(xué)研究中甲狀腺功能評(píng)估、內(nèi)分泌干擾物效應(yīng)分析、藥物干預(yù)實(shí)驗(yàn)中的激素水平監(jiān)測(cè)，或體外細(xì)胞模型中甲狀腺激素分泌機(jī)制研究；為探究甲狀腺相關(guān)代謝通路、疾病模型構(gòu)建及藥理學(xué)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

T3
種屬：

Ovis aries (Sheep)
樣本類(lèi)型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

0.5 ng/mL-8 ng/mL
靈敏度：

0.5 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheepT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	90-100
1:2	Average %	87
	Range %	85-96
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	82-99

回收率：

The recovery of sheep T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	85-102
EDTA plasma (n=4)	96	90-100

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.