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  4. Rat apolipoprotein E (Apo-E) ELISA Kit

Rat apolipoprotein E (Apo-E) ELISA Kit

  • 中文名稱：
    大鼠載脂蛋白E(Apo-E)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E09749r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠載脂蛋白E(Apo-E)酶聯(lián)免疫試劑盒（CSB-E09749r）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的APOE含量。APOE是一種與脂質(zhì)代謝和神經(jīng)系統(tǒng)密切相關(guān)的蛋白質(zhì)，其基因多態(tài)性是阿爾茨海默?。ˋD）的主要遺傳風險因素。研究顯示，APOE4等位基因與AD風險增加相關(guān)，其通過調(diào)節(jié)β-淀粉樣蛋白（Aβ）的聚集和清除、tau蛋白的磷酸化以及神經(jīng)炎癥等途徑影響AD的發(fā)病機制。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，靈敏度為15.6 ng/mL。適用于基礎科研中Apo - E表達水平檢測、藥物干預效果評估、疾病模型機制研究等場景，為心血管疾病、神經(jīng)退行性疾病等領(lǐng)域的體外研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ApoeApolipoprotein E ELISA kit; Apo-E ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell culture supernates
  • 檢測范圍：
    15.6 ng/mL-1000 ng/mL
  • 靈敏度：
    15.6 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-98  
    1:200 Average % 98  
    Range % 93-104  
    1:400 Average % 84  
    Range % 82-88  
    1:800 Average % 90  
    Range % 85-97  
  • 回收率：
    The recovery of rat Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-94  
    EDTA plasma (n=4) 94 90-99  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1000 0.067 0.065 0.066    
    500 0.121 0.125 0.123    
    250 0.213 0.204 0.209    
    125 0.386 0.355 0.371    
    62.5 0.604 0.590 0.597    
    31.2 0.811 0.858 0.835    
    15.6 1.109 1.116 1.113    
    0 2.155 2.214 2.185    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 最新研究進展：
        APOE是一種編碼APOE蛋白的基因，其蛋白質(zhì)產(chǎn)物APOE在多種生物學過程中發(fā)揮著重要作用。最新的研究表明，APOE基因突變與許多疾病的發(fā)生風險密切相關(guān)，例如阿爾茨海默病和冠狀動脈疾病等。此外，APOE蛋白還涉及到膽固醇代謝、神經(jīng)遞質(zhì)轉(zhuǎn)運和免疫反應等生理過程。
     
  • 功能：
    APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.
  • 基因功能參考文獻：
    1. Infusion of AngII did not lead to the formation of dissecting abdominal aortic aneurysms or atherosclerosis in ApoE-knockout rats. PMID: 29166645
    2. Results demonstrated that apolipoprotein E4 (ApoE4)exerted direct neurotoxicity and enhanced the neurotoxicity of Abeta1-40 on spatial cognitive function and hippocampal long term potentiation. This may partly elucidate the mechanism by which APOE4 allele exerted negative effects as a major genetic risk factor for developing Alzheimer's disease. PMID: 28356228
    3. renal denervation improves aortic distensibility and attenuated endothelial dysfunction in ApoE(-/-)-rats. PMID: 27277003
    4. The expression of ApoE and S-100 correlated with the progression of focal cerebral contusion. PMID: 26782580
    5. Apolipoprotein E-deficient rats develop atherosclerotic plaques in partially ligated carotid arteries. PMID: 26545012
    6. rats treated with KD (EXP2) showed a significant down-regulated expression of ZnT-3, MT-3, ApoE, clusterin, and ACAT-1 in hippocampus PMID: 25778834
    7. Findings suggest that endogenous apoE generates lower density HDL to produce more lipidated HDL using previously synthesized cholesterol through the interaction with ABCA1 in caveolin-1-rich domain of astrocytes PMID: 24814386
    8. Although rat apolipoprotein E sequence resembles apoE4, an isoform of apoE3, rat apoE displays the biophysical behavior of apoE3. PMID: 23103361
    9. A potential neuroprotective role of apolipoprotein E-containing lipoproteins through low density lipoprotein receptor-related protein 1 in normal tension glaucoma PMID: 22674573
    10. Lower expression of MMP-2 and MMP-9 is associated with the increased expression of apoE in the glomerulus PMID: 22207560
    11. ApoE-containing astrocyte lipoproteins exhibit the most robust interactions with Abeta. PMID: 22138302
    12. Apolipoproteins E3 exerts neurotrophic and synaptogenic effects in dorsal root ganglion cell cultures. PMID: 19414061
    13. LpE and LRP1 promote axonal extension, and suggest that lipids exported to LpE by ABCG1 are important for the enhancement of axonal extension mediated by LpE. PMID: 21040802
    14. ApoE may play a role in lipid rafts or synaptic structural plasticity by the regulation of its levels via dendritic localization and translation. PMID: 20456011
    15. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection. PMID: 19382209
    16. Although ApoE increases arterial wall accumulation of triglyceride-rich particles(TGRPs), it also reduces the penetration of TGRPs into the arterial wall--a novel antiatherogenic property. PMID: 12482838
    17. ApoE mRNA was expressed in astrocytes and macrophages, but not in neurons after focal cerebral ischemia PMID: 12870266
    18. Spatiotemporal changes of apolipoprotein E immunoreactivity and apolipoprotein E mRNA expression after transient middle cerebral artery occlusion in rat brain. PMID: 12898539
    19. The Apolipoprotein (apo) E is a protein involved in both lipid metabolism and neuroprotection and its expression system will be highly useful for probing the ability of rat apoE to mediate food intake in rats. PMID: 15866734
    20. Data indicate that the role of apoE4 in cholesterol homeostasis and apolipoprotein cell association is more pronounced in hippocampal neurons. PMID: 16376010
    21. Our studies suggest that AEC I is not just a simple barrier for gas exchange, but a functional cell that protects alveolar epithelium from injury. PMID: 16497717
    22. 24(S)-hydroxycholesterol induces apoE-mediated efflux of cholesterol in astrocytes via an LXR-controlled pathway PMID: 16524875
    23. ApoE expression is not altered during normal brain aging, but there may be a relationship between ApoE and IL-1beta transcription in the cerebral cortex PMID: 16802110
    24. LRP binds and endocytoses Abeta42 both directly and via apoE but endocytosed Abeta42 is not completely degraded and accumulates in intraneuronal lysosomes PMID: 17012232
    25. A naturally processed C-terminal ApoE peptide, Ep1.B, has anti-atherogenic activity indicating a role for this naturally metabolized peptide in vascular wound healing and lipid homeostasis PMID: 17126342
    26. treatment of primary rat mixed glial cell cultures with IL-1beta induced a significant increase in extracellular apoE protein; treatment of primary astrocyte and mixed glial cell cultures with TNF-alpha significantly reduced extracellular apoE PMID: 18288929
    27. mRNA expression of ApoE in arterial walls was not different between the controls and cerebral aneurysms. PMID: 18360691
    28. Data demonstrate that apoE suppresses food intake via a mechanism enhancing melanocortin signaling in the hypothalamus. PMID: 18559657
    29. The finding that hypothalamic apoE and food intake are positively associated during the normal circadian cycle as well as in the period of restricted feeding suggests that hypothalamic apoE is food-entrained and regulates food intake. PMID: 19362542
    30. Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin. PMID: 19717566

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  • 亞細胞定位：
    Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 數(shù)據(jù)庫鏈接：