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  4. Rat apolipoprotein H (Apo-H) ELISA Kit

Rat apolipoprotein H (Apo-H) ELISA Kit

  • 中文名稱：
    大鼠載脂蛋白H(Apo-H)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E10073r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠載脂蛋白H(Apo-H)酶聯(lián)免疫試劑盒（CSB-E10073r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOH含量。APOH（載脂蛋白H）是一種參與脂質(zhì)代謝的關(guān)鍵蛋白，在調(diào)節(jié)凝血和炎癥方面發(fā)揮著重要作用。研究表明，APOH與多種疾病有關(guān)，包括自身免疫性疾病和心血管疾病。其研究機制主要涉及APOH與帶負(fù)電物質(zhì)（如肝素和磷脂）的相互作用，以及其在防止內(nèi)在凝血級聯(lián)激活中的作用。試劑盒檢測范圍為28 ng/mL-1800 ng/mL，適用于大鼠模型中Apo-H的定量分析，可支持脂代謝研究、心血管疾病機制探索以及免疫相關(guān)實驗的開展，特別適用于基礎(chǔ)科研中涉及脂蛋白調(diào)控、血栓形成或免疫應(yīng)答機制的相關(guān)課題。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    ApohBeta-2-glycoprotein 1 ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    28 ng/mL-1800 ng/mL
  • 靈敏度：
    7 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %91
    Range %86-95
    1:400Average %102
    Range %97-107
    1:800Average %91
    Range %85-97
    1:1600Average %97
    Range %91-103
  • 回收率：
    The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)107101-110
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    18002.655 2.532 2.594 2.464
    9002.164 2.117 2.141 2.011
    4501.597 1.544 1.571 1.441
    2250.845 0.828 0.837 0.707
    1120.501 0.511 0.506 0.376
    560.307 0.300 0.304 0.174
    280.234 0.222 0.228 0.098
    00.131 0.129 0.130
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 組織特異性：
    Expressed by the liver and secreted in plasma.
  • 數(shù)據(jù)庫鏈接：

    UniGene: Rn.1824