Rat glycosaminoglycan/mucopolysaccharide ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠黏多糖/糖胺聚糖酶聯(lián)免疫試劑盒（CSB-EQ027297RA）為競爭法ELISA試劑盒，定量檢測血清、血漿、尿液樣本中的GAGs含量。GAGs即糖胺聚糖，在人體組織細(xì)胞中廣泛分布，參與眾多生理病理過程。研究發(fā)現(xiàn)，它與細(xì)胞黏附、遷移、增殖等活動密切相關(guān)，還在腫瘤發(fā)生發(fā)展、炎癥反應(yīng)等機(jī)制里發(fā)揮作用，是疾病治療靶點以及藥物研發(fā)的重要研究對象。試劑盒檢測范圍為6.25 ng/mL-100 ng/mL，在代謝性疾病模型研究、藥物干預(yù)效果評估及組織修復(fù)機(jī)制探索中具有重要應(yīng)用價值；可廣泛應(yīng)用于大鼠疾病模型（如黏多糖貯積癥、關(guān)節(jié)炎等）的病理機(jī)制研究、代謝調(diào)控實驗及生物標(biāo)志物篩選等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

GAGs
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, urine
檢測范圍：

6.25 ng/mL-100 ng/mL
靈敏度：

3.125 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat glycosaminoglycan in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-97
1:200	Average %	98
	Range %	93-104
1:400	Average %	85
	Range %	81-88
1:800	Average %	88
	Range %	83-94

回收率：

The recovery of rat glycosaminoglycan spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	92	88-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.