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  4. Rat heparan sulfate proteoglycan 2 (HSPG2) ELISA kit

Rat heparan sulfate proteoglycan 2 (HSPG2) ELISA kit

  • 中文名稱：
    大鼠硫酸乙酰肝素蛋白多糖2(HSPG2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL010868RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠硫酸乙酰肝素蛋白多糖2(HSPG2)酶聯(lián)免疫試劑盒（CSB-EL010868RA）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、支氣管肺泡灌洗液、尿液、組織勻漿樣本中的HSPG2含量。HSPG2 即硫酸乙酰肝素蛋白聚糖 2，是細(xì)胞外基質(zhì)的重要成分。它參與細(xì)胞黏附、增殖等過程。研究機(jī)制方面，其異常表達(dá)與腫瘤生長(zhǎng)、侵襲和轉(zhuǎn)移密切相關(guān)，還涉及多種疾病的病理進(jìn)程，是頗具潛力的疾病診斷與治療靶點(diǎn)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，特別適用于體外實(shí)驗(yàn)?zāi)Ｐ椭蠬SPG2表達(dá)的動(dòng)態(tài)監(jiān)測(cè)，例如細(xì)胞外基質(zhì)相互作用研究、血管生成機(jī)制探索或組織修復(fù)模型的建立。其寬泛的樣本兼容性為科研人員提供了靈活選擇實(shí)驗(yàn)樣本的條件，支持從體液到組織水平的系統(tǒng)性研究。為分子生物學(xué)、細(xì)胞生物學(xué)及基礎(chǔ)醫(yī)學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    RP11-132G19.2, PLC, PRCAN, SJA, SJS, SJS1, Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia)|endorepellin (domain V region)|heparan sulfate proteoglycan of basement membrane|perlecan proteogly
  • 縮寫：
    HSPG2
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, bronchoalveolar lavage fluid, urine, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HSPG2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %83
    Range %80-86
    1:400Average %97
    Range %93-101
    1:800Average %91
    Range %85-97
    1:1600Average %100
    Range %93-107
  • 回收率：
    The recovery of rat HSPG2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-93
    EDTA plasma (n=4)104100-108
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.766 2.798 2.782 2.680
    5002.277 2.286 2.282 2.180
    2501.593 1.562 1.578 1.476
    1250.996 0.963 0.980 0.878
    62.50.533 0.542 0.538 0.436
    31.20.384 0.368 0.376 0.274
    15.60.271 0.291 0.281 0.179
    00.101 0.103 0.102
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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