Rat proteoglycan 4(PRG4) ELISA kit
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中文名稱(chēng):大鼠蛋白聚糖4(PRG4)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E14125r
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:大鼠蛋白聚糖4(PRG4)酶聯(lián)免疫試劑盒(CSB-E14125r)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PRG4含量。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,科研人員可將其應(yīng)用于大鼠關(guān)節(jié)炎模型構(gòu)建、軟骨退行性病變機(jī)制探索、關(guān)節(jié)液生物標(biāo)志物分析等領(lǐng)域,也可用于評(píng)估運(yùn)動(dòng)損傷或藥物治療對(duì)關(guān)節(jié)內(nèi)PRG4表達(dá)的影響。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名:Plppr2; Lppr2; Prg4; Phospholipid phosphatase-related protein type 2; Lipid phosphate phosphatase-related protein type 2; Plasticity-related gene 4 protein; PRG-4
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縮寫(xiě):PRG4
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Uniprot No.:
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種屬:Rattus norvegicus (Rat)
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樣本類(lèi)型:serum, plasma, tissue homogenates
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檢測(cè)范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.39 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Others
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線(xiàn)性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat PRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 88-95 1:2 Average % 96 Range % 92-101 1:4 Average % 102 Range % 99-103 1:8 Average % 87 Range % 82-93 -
回收率:
The recovery of rat PRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 87-94 EDTA plasma (n=4) 101 98-105 -
標(biāo)準(zhǔn)曲線(xiàn):
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.721 2.888 2.805 2.610 50 1.806 1.765 1.786 1.591 25 1.154 1.140 1.147 0.952 12.5 0.702 0.678 0.690 0.495 6.25 0.512 0.520 0.516 0.321 3.12 0.368 0.362 0.365 0.170 1.56 0.264 0.256 0.260 0.065 0 0.192 0.198 0.195 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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亞細(xì)胞定位:Membrane; Multi-pass membrane protein.
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蛋白家族:PA-phosphatase related phosphoesterase family
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數(shù)據(jù)庫(kù)鏈接:
STRING: 10116.ENSRNOP00000016790
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