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  4. Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

  • 中文名稱：
    大鼠正常T細(xì)胞表達(dá)和分泌因子/趨化因子5(RANTES/CCL5)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07398r-IS
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠正常T細(xì)胞表達(dá)和分泌因子/趨化因子5(RANTES/CCL5)酶聯(lián)免疫試劑盒（CSB-E07398r-IS）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的RANTES/CCL5含量。RANTES/CCL5屬于趨化因子，在免疫反應(yīng)等生理病理過程中起重要作用。研究發(fā)現(xiàn)它可趨化多種免疫細(xì)胞，參與炎癥反應(yīng)、腫瘤轉(zhuǎn)移等進(jìn)程。其作用機(jī)制主要是與相應(yīng)受體結(jié)合，激活下游信號(hào)通路，引發(fā)系列細(xì)胞反應(yīng)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，靈敏度為7.8 pg/mL。適用于多種基礎(chǔ)科研場(chǎng)景：包括但不限于炎癥性疾病機(jī)制研究（如關(guān)節(jié)炎模型）、免疫應(yīng)答動(dòng)態(tài)分析、病毒感染相關(guān)趨化因子調(diào)控實(shí)驗(yàn)（如HIV相關(guān)研究），以及藥物開發(fā)過程中抗炎或免疫調(diào)節(jié)化合物的體外效果評(píng)估。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES/CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:10

    Average %

    99

    Range %

    92-105

    1:20

    Average %

    93

    Range %

    89-98

    1:40

    Average %

    101

    Range %

    96-106

    1:80

    Average %

    90

    Range %

    85-96

  • 回收率：

    The recovery of rat RANTES/CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    96

    89-102

    EDTA plasma (n=4)

    91

    84-98

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    2.863

    2.914

    2.888

    2.738

    500

    1.685

    1.617

    1.651

    1.501

    250

    0.772

    0.738

    0.755

    0.605

    125

    0.445

    0.429

    0.437

    0.287

    62.5

    0.313

    0.315

    0.314

    0.164

    31.2

    0.225

    0.233

    0.229

    0.079

    15.6

    0.196

    0.189

    0.192

    0.042

    0

    0.152

    0.148

    0.150

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數(shù)據(jù)庫鏈接：