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  4. Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

  • 中文名稱：
    大鼠可溶性CD14(sCD14)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E11178r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠可溶性CD14(sCD14)酶聯(lián)免疫試劑盒（CSB-E11178r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CD14含量。CD14為一種細(xì)胞表面糖蛋白，是天然免疫中重要模式識別受體。它能識別細(xì)菌脂多糖等病原體相關(guān)分子模式。研究機制方面，可通過與配體結(jié)合，激活下游信號通路，促使細(xì)胞產(chǎn)生炎性因子等，在抗感染免疫等方面有重要作用。試劑盒檢測范圍為28 ng/mL-1800 ng/mL，常被用于研究感染性疾病和炎癥相關(guān)機制；適用于研究感染模型、炎癥性疾病或免疫調(diào)控過程中 sCD14 的動態(tài)變化，為探索宿主免疫應(yīng)答機制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Cd14Monocyte differentiation antigen CD14 ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit; CD antigen CD14 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    28 ng/mL-1800 ng/mL
  • 靈敏度：
    7 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %86-100
    1:2Average %99
    Range %90-103
    1:4Average %89
    Range %83-92
    1:8Average %95
    Range %84-98
  • 回收率：
    The recovery of rat sCD14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10294-108
    EDTA plasma (n=4)9689-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    18002.530 2.401 2.466 2.283
    9002.066 1.986 2.026 1.843
    4501.311 1.356 1.334 1.151
    2250.759 0.779 0.769 0.586
    112.50.456 0.478 0.467 0.284
    560.352 0.344 0.348 0.165
    280.271 0.263 0.267 0.084
    00.181 0.185 0.183
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).
  • 基因功能參考文獻：
    1. This study shows that sCD14 impairs contractile function and insulin action in primary adult rat cardiomyocytes. PMID: 27816522
    2. CD 14 mRNA expression increased significantly after five days of bile duct ligation in liver, lung, spleen, and ileum. PMID: 25093541
    3. biliary drainage affects expression of inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats PMID: 23613625
    4. Burn injury-induced mitochondrial damage in cardiomyocytes was prevented in CD14 knockout mice. Burn injury produces CD14-dependent mitochondrial damage via oxidative stress in myocardium. PMID: 20348223
    5. Acute cerebral hemorrhage results in endotoxemia and widespread increases in CD14 and IL-1beta expression. PMID: 19534684
    6. Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia. PMID: 12046090
    7. Increase of CD14 mRNA expression may result in greater sensitivity to endotoxin and thus lead to liver injury. PMID: 12113681
    8. Although microglial-derived CD14 does not contribute to early events triggered by intrastriatal endotoxin treatment, it modulates the duration of inflammation within the cerebral tissue. PMID: 12218159
    9. Ethanol administration led to a significant synthesis of endotoxin receptor CD14 protein and its gene expression in Kupffer cells, which maybe result in the pathological changes of liver tissue and hepatic functional damages. PMID: 12632533
    10. the CD14 antigen system appeared to be an early trigger for Kupffer cell activation followed by induction of inflammatory cytokine interleukin-1beta synthesis leading to hepatic injury after partial hepatectomy PMID: 14572767
    11. CD14 glycoprotein is present on the cell membranes of vascular smooth muscle cells of rat aorta, where it acts as a lipopolysaccharide receptor. PMID: 15153652

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  • 亞細(xì)胞定位：
    Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus.
  • 組織特異性：
    Detected in macrophages and peripheral blood monocytes.
  • 數(shù)據(jù)庫鏈接：