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  4. Rat soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

Rat soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

  • 中文名稱：
    大鼠可溶性血小板內(nèi)皮細(xì)胞粘附分子1(sPECAM-1/sCD31)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08134r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3900/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠可溶性血小板內(nèi)皮細(xì)胞粘附分子1(sPECAM-1/sCD31)酶聯(lián)免疫試劑盒（CSB-E08134r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PECAM1含量。PECAM1即血小板內(nèi)皮細(xì)胞粘附分子-1，在血管生成、白細(xì)胞轉(zhuǎn)運(yùn)等生理過(guò)程中發(fā)揮重要作用。研究機(jī)制方面，它參與細(xì)胞間黏附及信號(hào)傳導(dǎo)，其異常表達(dá)與多種疾病如腫瘤、心血管疾病相關(guān)。深入研究有助于了解疾病發(fā)病機(jī)制并尋找潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為3.12 ng/mL-200 ng/mL，適用于心血管疾病動(dòng)物模型分析、炎癥機(jī)制研究以及藥物干預(yù)效果評(píng)價(jià)等科研領(lǐng)域，為探索血管內(nèi)皮功能異常相關(guān)疾病提供可靠的實(shí)驗(yàn)工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名：
    Pecam1 ELISA Kit; Pecam ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit
  • 縮寫(xiě)：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 86-95    
    1:2 Average % 102    
    Range % 97-107    
    1:4 Average % 91    
    Range % 85-97    
    1:8 Average % 97    
    Range % 91-103    
  • 回收率：
    The recovery of rat sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 89-98    
    EDTA plasma (n=4) 97 90-100    
                   
                   
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    200 2.468 2.554 2.511 2.372    
    100 1.656 1.732 1.694 1.555    
    50 1.033 1.067 1.050 0.911    
    25 0.591 0.611 0.601 0.462    
    12.5 0.380 0.398 0.389 0.250    
    6.25 0.278 0.286 0.282 0.143    
    3.12 0.203 0.207 0.205 0.066    
    0 0.138 0.139 0.139 　    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.
  • 基因功能參考文獻(xiàn)：
    1. Circulating PECAM-1 is a promising biomarker for the detection and monitoring of ventilator-induced lung injury. PMID: 24588994
    2. Shock wave therapy leads to mobilization of endogenous CD31/CD34-positive endothelial cells. PMID: 23395097
    3. PECAM-1 expression on PMNs is in a converse way between pancreatic microcirculation and peripheral circulation in acute edematous pancreatitis PMID: 14599961
    4. A reverse expression of PECAM-1 on neutrophils was found between peripheral circulation and pancreatic microcirculation, so inhibition of PECAM-1 expression may improve the pathological change of acute edematous pancreatitis. PMID: 15655817
    5. expression pattern of PECAM & VE-cadherin at endothelial cell junctions in adult initial lymphatic network; results suggest that primary valves are associated with discontinuous expression of endothelial junction molecules PMID: 17935476

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  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Membrane raft. Cell junction.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: rno:29583

    STRING: 10116.ENSRNOP00000044403

    UniGene: Rn.1878