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Sheep Vasopressin-neurophysin 2-copeptin(AVP) ELISA kit

  • 中文名稱:
    綿羊加壓素-神經(jīng)垂體素運載蛋白-2-和肽素(AVP)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-EL002466SH
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    綿羊加壓素-神經(jīng)垂體素運載蛋白-2-和肽素(AVP)酶聯(lián)免疫試劑盒(CSB-EL002466SH)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的AVP含量。AVP即精氨酸加壓素,是一種具有多種生理功能的神經(jīng)肽。其參與調(diào)節(jié)水鹽平衡、血壓穩(wěn)定等。在研究機制上,它通過與特定受體結(jié)合,調(diào)控細胞內(nèi)信號傳導(dǎo)通路,影響腎臟、心血管等多種組織器官功能,相關(guān)研究為治療相關(guān)疾病提供了方向。試劑盒檢測范圍為1.56 pg/mL-100 pg/mL,適用于科研領(lǐng)域探究綿羊心血管系統(tǒng)、腎臟功能或應(yīng)激生理機制的相關(guān)研究,特別是在動物模型的水代謝異常、抗利尿激素相關(guān)調(diào)控機制等方向具有重要應(yīng)用價值,為神經(jīng)內(nèi)分泌領(lǐng)域的分子機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
  • 縮寫:
  • 種屬:
    Ovis aries (Sheep)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 pg/mL-100 pg/mL
  • 靈敏度:
    0.39 pg/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-94
    1:2Average %101
    Range %95-105
    1:4Average %92
    Range %87-95
    1:8Average %98
    Range %94-102
  • 回收率:
    The recovery of sheep AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9286-97
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.500 2.345 2.423 2.296
    501.922 1.889 1.906 1.779
    251.224 1.253 1.239 1.112
    12.50.755 0.776 0.766 0.639
    6.250.445 0.464 0.455 0.328
    3.120.316 0.303 0.310 0.183
    1.560.216 0.211 0.214 0.087
    00.128 0.126 0.127
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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