Sheep neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊中性粒細胞明膠酶相關(guān)脂質(zhì)運載蛋白(NGAL)酶聯(lián)免疫試劑盒（CSB-E13392Sh）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NGAL含量。NGAL即中性粒細胞明膠酶相關(guān)脂質(zhì)運載蛋白，是一種小分子分泌型蛋白。作為新興靶點，其與細胞增殖、凋亡、遷移等相關(guān)。研究機制主要圍繞它在炎癥、腫瘤等疾病中的表達變化，以及與其他分子相互作用對疾病進程的影響。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，在綿羊疾病模型研究中具有重要價值；適用于綿羊炎癥機制研究、腎臟疾病動物模型建立、藥物干預(yù)效果評價等科研領(lǐng)域；為實驗室開展NGAL相關(guān)病理生理研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

NGAL
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	92
	Range %	86-95
1:400	Average %	103
	Range %	99-105
1:800	Average %	94
	Range %	88-98
1:1600	Average %	99
	Range %	94-105

回收率：

The recovery of sheep NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-97
EDTA plasma (n=4)	95	88-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.