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  4. CYC1 Monoclonal Antibody

CYC1 Monoclonal Antibody

  • 中文名稱：
    CYC1鼠單克隆抗體
  • 貨號：
    CSB-MA006327A0m
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: CYC1 antibody at 1:1000
      Lane 1: Hela whole cell lysate
      Lane 2: HepG2 whole cell lysate
      Lane 3: MCF-7 whole cell lysate
      Lane 4: K562 whole cell lysate
      Lane 5: U251 whole cell lysate
      Lane 6: A549 whole cell lysate
      Lane 7: NIH/3T3 whole cell lysate
      Lane 8: PC-3 whole cell lysate
      Secondary
      Goat polyclonal to Mouse IgG at 1/20000 dilution
      Predicted band size: 35 KDa
      Observed band size: 35 KDa
      Exposure time: 5min
    • Overlay Peak curve showing HepG2 cells stained with CSB-MA006327A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    本產(chǎn)品為針對細胞色素c1（CYC1）靶點研發(fā)的小鼠源單克隆抗體（代碼CUSABIO貨號：CSB-MA006327A0m），適用于人源與鼠源樣本的ELISA、Western Blot及流式細胞分析。CYC1作為線粒體電子傳遞鏈復(fù)合體III的核心亞基，在ATP合成與細胞能量代謝中發(fā)揮關(guān)鍵作用，其表達水平與細胞凋亡調(diào)控密切相關(guān)。該抗體經(jīng)嚴(yán)格驗證具有高特異性，可精準(zhǔn)識別天然構(gòu)象的CYC1蛋白，適用于線粒體功能研究、代謝疾病機制探索及氧化磷酸化通路分析等科研領(lǐng)域。實驗數(shù)據(jù)顯示其能有效應(yīng)用于多種樣本基質(zhì)檢測，例如通過Western Blot技術(shù)檢測肝組織或心肌細胞裂解液中的CYC1表達譜，或結(jié)合流式細胞術(shù)分析腫瘤細胞線粒體膜蛋白動態(tài)變化。本產(chǎn)品為人鼠交叉反應(yīng)性抗體，可為心血管疾病、神經(jīng)退行性疾病及癌癥能量代謝研究提供可靠工具，滿足細胞生物學(xué)、分子病理學(xué)等基礎(chǔ)研究的實驗需求。關(guān)鍵詞：CYC1單克隆抗體、線粒體復(fù)合體III、細胞色素c1檢測、人鼠交叉反應(yīng)、ATP合成研究用抗體、Western Blot專用抗體。
  • 產(chǎn)品名稱：
    Mouse anti-Homo sapiens (Human) CYC1 Monoclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    Cytochrome c1, heme protein, mitochondrial (EC:7.1.1.8); Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; Cytochrome c-1
  • 宿主：
    Mouse
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    Recombinant Human Cytochrome c1 protein (85-325AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    IgG1
  • 純化方式：
    >95%, Protein A purified
  • 克隆號：
    27E12D11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.
  • 基因功能參考文獻：
    1. these results indicate that CYC1 plays important roles in cell proliferation and comedo necrosis through the elevated oxidative phosphorylation activity in human ductal carcinoma in situ PMID: 28394473
    2. results indicate that miR-661 plays a tumor suppressor role in OS mediated by the downregulation of CYC1, suggesting a potential mechanism underlying cell death resistance in Osteosarcoma. PMID: 28391262
    3. CYC1 promoted tumor metastasis via suppressing activation of AMPK and contributed to tumor growth via facilitating production of ATP. PMID: 27239088
    4. FASL, granzyme B, and cytochrome c blood expression reflects breast cancer progression and response to therapy. (Review) PMID: 27117663
    5. our results provide evidence that CYC1 plays an important role in OS tumorigenesis, and modulation of CYC1 may be an effective strategy to potentiate OS to apoptotic induction by TRAIL. PMID: 25562155
    6. Mutations in CYC1 cause insulin-responsive hyperglycemia. PMID: 23910460
    7. HeLa cells expressing non-cleavable cytochrome c1 maintain mitochondrial morphology and functions. PMID: 21577235
    8. Decreased electron Transport Complex IV activity is associated with ulcerative colitis. PMID: 20440543
    9. Addition of H202 to tendon fibroblasts in vitro resulted in oxidative stress-induced apoptosis and the release of CYC1. PMID: 12788227
    10. the Ca(2+) spike, mitochondrial Bcl-2 presensitization, and subsequent Delta psi(m) transition, Bax translocation, and ROS generation are important upstream signals for cytochrome c release upon stimulation by a Bcl-2 inhibitor. PMID: 14966123
    11. Sensitivity of Cyt c detection in -linked immunosorbent assay is reduced in the presence of serum leucine-rich alpha-2-glycoprotein-1. PMID: 16699948

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  • 相關(guān)疾?。?/div>
    Mitochondrial complex III deficiency, nuclear 6 (MC3DN6)
  • 亞細胞定位：
    Mitochondrion inner membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome c family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2579

    OMIM: 123980

    KEGG: hsa:1537

    STRING: 9606.ENSP00000317159

    UniGene: Hs.289271