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  4. TMEM192 Antibody

TMEM192 Antibody

  • 中文名稱：
    TMEM192兔多克隆抗體
  • 貨號(hào)：
    CSB-PA023784LA01HU
  • 規(guī)格：
    ￥440
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
      All lanes: TMEM192 antibody at 3.2µg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 31 kDa
      Observed band size: 31 kDa
    • IHC image of CSB-PA023784LA01HU diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-PA023784LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-PA023784LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunoprecipitating TMEM192 in Jurkat whole cell lysate
      Lane 1: Rabbit control IgG (1µg) instead of CSB-PA023784LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-PA023784LA01HU (6µg) + Jurkat whole cell lysate (500µg)
      Lane 3: Jurkat whole cell lysate (10µg)
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品名稱：
    Rabbit anti-Homo sapiens (Human) TMEM192 Polyclonal antibody
  • Uniprot No.：
  • 基因名：
    TMEM192
  • 別名：
    TMEM192; Transmembrane protein 192
  • 宿主：
    Rabbit
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human Transmembrane protein 192 protein (193-271AA)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated

    本頁面中的產(chǎn)品，TMEM192 Antibody (CSB-PA023784LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于TMEM192 Antibody，我們還提供其他標(biāo)記。見下表：

    可提供標(biāo)記
    標(biāo)記方式 貨號(hào) 產(chǎn)品名稱 應(yīng)用
    HRP CSB-PA023784LB01HU TMEM192 Antibody, HRP conjugated ELISA
    FITC CSB-PA023784LC01HU TMEM192 Antibody, FITC conjugated
    Biotin CSB-PA023784LD01HU TMEM192 Antibody, Biotin conjugated ELISA
  • 克隆類型：
    Polyclonal
  • 抗體亞型：
    IgG
  • 純化方式：
    >95%, Protein G purified
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:200-1:500
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Authors found that transmembrane protein 192 (TMEM192) interacted with TIG1. Authors also found that both TIG1A and TIG1B isoforms interacted and co-localized with TMEM192 in HtTA cervical cancer cells. The expression of TIG1 induced the expression of autophagy-related proteins. PMID: 27989102
    2. TMEM192 deficiency can induce autophagy in tumor cells, and can further activate apoptosis by the mitochondrial pathway through autophagy. PMID: 22736246
    3. TMEM192 was found to be strongly expressed in human kidney, liver, lung and pancreas tissue. The widespread tissue distribution could suggest an important role of TMEM192 for lysosomal function. PMID: 20370317
  • 亞細(xì)胞定位：
    Lysosome membrane; Multi-pass membrane protein. Late endosome.
  • 蛋白家族：
    TMEM192 family
  • 組織特異性：
    Strongly expressed in kidney, liver, lung and pancreas.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 26775

    KEGG: hsa:201931

    STRING: 9606.ENSP00000305069

    UniGene: Hs.704511