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CLDN18 Recombinant Monoclonal Antibody

  • 中文名稱：
    CLDN18重組抗體
  • 貨號：
    CSB-RA005498A2HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Untransfected KATO3 cells surface (green line), transfected Human CLDN18.2 KATO3 stable cells surface (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells surface (blue line) were stained with anti-CLDN18.2 antibody (2μg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
    • The Binding Activity of Human CLDN18.2 with Anti-CLDN18 recombinant antibody.
      Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/mL can bind Anti-CLDN18 recombinant antibody, the EC50 is 2.947-8.782 ng/mL.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CLDN18重組單克隆抗體（CSB-RA005498A2HU）是針對緊密連接蛋白CLDN18開發(fā)的高特異性科研工具。CLDN18是維持上皮細胞屏障功能的關(guān)鍵分子，在胃黏膜組織特異性表達，參與調(diào)控細胞旁通透性及細胞極性，其異常表達與胃部疾病及部分腫瘤微環(huán)境調(diào)控相關(guān)。本抗體經(jīng)ELISA和流式細胞術(shù)（FC）雙重驗證，在FC應(yīng)用中推薦使用1:50-1:200的稀釋范圍，實驗者可結(jié)合具體樣本特性優(yōu)化條件。該重組抗體采用單克隆技術(shù)制備，具有高批次間一致性，有效降低實驗重復(fù)性風(fēng)險，適用于細胞膜表面靶標檢測及定量分析。在科研場景中，可應(yīng)用于：①研究上皮屏障功能異常與消化系統(tǒng)疾病的關(guān)系；②分析腫瘤細胞極性改變對微環(huán)境的影響；③構(gòu)建胃黏膜相關(guān)疾病模型中CLDN18的動態(tài)表達譜；④探索細胞連接復(fù)合體在組織穩(wěn)態(tài)維持中的作用機制。本產(chǎn)品為無載體蛋白液體劑型，開蓋即用，建議配合同源對照抗體進行實驗設(shè)計，以滿足細胞表面標記檢測的精準需求。
  • Uniprot No.：
  • 基因名：
  • 別名：
    CLDN18; UNQ778/PRO1572; Claudin-18
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human CLDN18 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    hIgG1
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    25E3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 基因功能參考文獻：
    1. In this study, we found downregulation of miR-767-3p and upregulation of CLDN18 in lung adenocarcinoma tissue and cell lines. PMID: 29169410
    2. CDH17 and CLDN18 are useful target molecules. Their coupling can aid in the comprehensive detection and localization of gastric cancer metastases in vivo to overcome challenges associated with intratumoral heterogeneity. PMID: 27580354
    3. Bile duct adenocarcinoma cells overexpress claudin-18 via the EGFR/RAS/ERK pathway, contributing to cell proliferation and invasion. PMID: 28624624
    4. The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 downregulates claudin-18. These data also suggest that the loss of claudin-18 is associated with increased sensitization to aeroantigens and airway responsiveness PMID: 27215490
    5. suggest that the reduction of CLDN5, 7, and 18 expression loses the suppressive ability of interaction between PDK1 and Akt and causes sustained phosphorylation of Akt, resulting in the disordered proliferation in lung squamous carcinoma cells PMID: 27884700
    6. Data suggest that claudin-18 suppresses the abnormal proliferation and motility of lung epithelial cells mediated by inhibition of phosphorylation of pyruvate dehydrogenase kinase isoform 1 (PDK1) and proto-oncogene protein c-akt (Akt). PMID: 26919807
    7. Human fetal lungs at 23-24 weeks gestational age, the highest-risk period for developing bronchopulmonary dysplasia, a disease of impaired alveolarization, had significantly lower CLDN18 expression relative to postnatal lungs. PMID: 24787463
    8. Evaluated expression of claudins in gastric cancer and determined their significance for patient outcome. Claudin-3 and claudin-7 were expressed in 25.4% and 29.9% of gastric cancer tissues; 51.5% of gastric cancer tissues had reduced claudin-18. PMID: 24333468
    9. High levels of CLDN18 are associated with non-small-cell lung cancer. PMID: 24710653
    10. Downregulation of miR-1303 can inhibit proliferation, migration and invasion of gastric cancer cells by targeting CLDN18. PMID: 24647998
    11. Claudin-18 positivity is a specific phenotype that is characteristic of intestinal-type Mucinous borderline tumours of the ovary PMID: 23905715
    12. Down-regulation of claudin-18 is associated with the proliferative and invasive potential of gastric cancer. PMID: 24073219
    13. rate of CLDN18.2 positivity is high in pancreatic neoplasms whereby the expression is not limited to the primaries but is also maintained upon metastasis PMID: 23900716
    14. Claudin 10/18 are most commonly expressed in lung adenocarcinomas. Female patients and non-smokers express these claudins more commonly suggesting that they may play a part in the carcinogenesis of tobacco unrelated carcinoma. PMID: 22076167
    15. Claudin 18 (a marker for early carcinogenesis) is commonly expressed in precursor lesions of pancreatic ductal adenocarcinomas. Activation of the protein kinase C pathway might be involved in claudin 18 expression associated with carcinogenesis. PMID: 21832145
    16. Cldn18 is primarily regulated at the transcriptional level via specific protein kinase C signaling pathways and modified by DNA methylation PMID: 21381080
    17. These results suggest that CLDN18 may play an important role in biliary carcinogenesis. PMID: 21607649
    18. High claudin 18 is associated with intraductal papillary mucinous neoplasms of the pancreas. PMID: 21206985
    19. We revealed that claudin-18 expression correlates with poor survival in patients with colorectal cancer and is associated with the gastric phenotype. PMID: 20846265
    20. Loss of claudin expression may enhance the grade of malignancy of gastric cancer in vivo. PMID: 17459057
    21. We conclude that Cldn-18 is the dominant claudin in the TJ of SCE and propose that the change from a Cldn-18-deficient TJ in SqE to a Cldn-18-rich TJ in SCE contributes to the greater acid resistance of BE. PMID: 17932229
    22. Indicate that the PKC/MAPK/AP-1 dependent pathway regulates claudin-18a2 expression in gastric cells. PMID: 18032479
    23. Claudin 18 and annexin A8 are frequently highly overexpressed in infiltrating ductal adenocarcinomas. PMID: 18223320
    24. Claudin 18 staining can aid in diagnosis of gastrointestinal signet ring cell carcinoma. PMID: 18580680
    25. Increased expression of claudin-18 is associated with colitis. PMID: 18831034
    26. CLDN18.2 as a novel, highly attractive pan-cancer target for the antibody therapy of epithelial tumors. PMID: 19047087

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  • 亞細胞定位：
    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Claudin family
  • 組織特異性：
    Isoform A1: Expression is restricted to the lung. Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine c
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2039

    OMIM: 609210

    KEGG: hsa:51208

    STRING: 9606.ENSP00000183605

    UniGene: Hs.655324