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Claudin-18.2 Recombinant Monoclonal Antibody

  • 中文名稱:
    Claudin-18.2重組抗體
  • 貨號(hào):
    CSB-RA005498A1HU
  • 規(guī)格:
    ¥1320
  • 圖片:
    • Untransfected KATO3 cells surface (green line), transfected Human CLDN18.2 KATO3 stable cells surface (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells surface (blue line) were stained with anti-CLDN18.2 antibody (2μg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
    • The Binding Activity of Human CLDN18.2 with Anti-CLDN18.2 recombinant antibody.
      Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/mL can bind Anti-CLDN18.2 recombinant antibody, the EC50 is 6.554-27.87 ng/mL.
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    CSB-RA005498A1HU Claudin-18.2重組單克隆抗體是針對(duì)緊密連接蛋白家族成員Claudin-18.2研發(fā)的高特異性科研試劑。該靶點(diǎn)通過調(diào)控上皮細(xì)胞間緊密連接參與屏障功能維持,其亞型Claudin-18.2在胃癌、胰腺癌等消化道腫瘤中呈現(xiàn)特異性高表達(dá),成為腫瘤生物學(xué)研究的重要生物標(biāo)志物。本抗體經(jīng)ELISA和流式細(xì)胞術(shù)(FC)雙重驗(yàn)證,在FC應(yīng)用中推薦使用1:50-1:200的稀釋范圍,可精準(zhǔn)識(shí)別天然構(gòu)象的膜表面抗原,適用于活細(xì)胞或固定化樣本的定量檢測(cè)。該產(chǎn)品憑借優(yōu)異的批間一致性和低交叉反應(yīng)特性,可支持腫瘤細(xì)胞表面標(biāo)記物分析、靶向藥物體外篩選模型構(gòu)建以及腫瘤微環(huán)境中細(xì)胞亞群分選等研究場(chǎng)景,為探索Claudin-18.2在腫瘤發(fā)生、轉(zhuǎn)移及耐藥機(jī)制中的功能提供可靠工具,助力抗腫瘤藥物開發(fā)及轉(zhuǎn)化醫(yī)學(xué)研究。本產(chǎn)品嚴(yán)格限定于體外實(shí)驗(yàn)及基礎(chǔ)科研用途。
  • Uniprot No.:
  • 基因名:
  • 別名:
    Claudin-18, CLDN18
  • 反應(yīng)種屬:
    Human
  • 免疫原:
    Recombinant Human Claudin-18.2 protein
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標(biāo)記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    hIgG1
  • 純化方式:
    Affinity-chromatography
  • 克隆號(hào):
    9C2
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式:
    Liquid
  • 應(yīng)用范圍:
    ELISA, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols:
  • 儲(chǔ)存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途:
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

平均分:
4.5分 - 2 個(gè)評(píng)價(jià)

實(shí)驗(yàn)應(yīng)用:IHC, FC, ELISA

樣品信息: 人胃黏膜細(xì)胞

稀釋比:1:100

產(chǎn)品評(píng)價(jià): 我使用CSB-RA005498A1HU的抗體,進(jìn)行ELISA和WB檢測(cè)人的細(xì)胞,WB檢測(cè)結(jié)果條帶較單一,檢測(cè)條帶大小與預(yù)期的一致,ELISA檢測(cè)EC50 為2.623-14.06 ng/mL。抗體特異性好,靈敏度高。

By 彭老師

實(shí)驗(yàn)應(yīng)用:FC

樣品信息:人來源細(xì)胞

稀釋比:1.100

產(chǎn)品評(píng)價(jià): 正常流式過程。

By 付老師