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GYPA Recombinant Monoclonal Antibody

  • 中文名稱：
    GYPA重組抗體
  • 貨號：
    CSB-RA257220A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Overlay Peak curve showing K562 cells surface stained with CSB-RA257220A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA257220A0HU重組單克隆抗體是針對人類血型糖蛋白A（GYPA）開發(fā)的高特異性科研工具。GYPA是紅細胞膜表面重要的跨膜糖蛋白，作為MN血型抗原的主要載體，不僅參與細胞間相互作用和膜結(jié)構(gòu)穩(wěn)定，還在病原體識別（如瘧原蟲入侵）等生物學(xué)過程中發(fā)揮關(guān)鍵作用。本抗體經(jīng)ELISA和流式細胞術(shù)（FC）嚴格驗證，在FC應(yīng)用中推薦使用1:50-1:200稀釋比例，能有效識別天然構(gòu)象的GYPA抗原，具有優(yōu)異的批次穩(wěn)定性和低交叉反應(yīng)性。適用于紅細胞膜蛋白功能研究、血型抗原表達分析、血液系統(tǒng)疾病模型建立以及感染性疾病機制探索等科研領(lǐng)域，特別適用于需要高靈敏度檢測細胞表面抗原表達的流式細胞分析實驗，為血液學(xué)、免疫學(xué)及病原體-宿主相互作用研究提供可靠試劑支持。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Glycophorin-A (MN sialoglycoprotein) (PAS-2) (Sialoglycoprotein alpha) (CD antigen CD235a), GYPA, GPA
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human GYPA
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    27A8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
  • 基因功能參考文獻：
    1. Cell viability and surface expression of transferrin receptor (CD71) and glycophorin A (GPA) were analyzed before and after re-culture by flow cytometry. These studies show differential sensitivities of these surface proteins on K562 cells to proteases, and suggest molecular mechanisms of transmembrane protein transport and cycling. PMID: 28980921
    2. a complex structural rearrangement involving the loss of GYPB and gain of two GYPB-A hybrid genes reduces the risk of severe malaria by 40% and has recently increased in frequency in parts of Kenya PMID: 28522690
    3. The authors show that Plasmodium falciparum EBA-175 mediates substantial changes in the deformability of erythrocytes by binding to glycophorin A and activating a phosphorylation cascade that includes erythrocyte cytoskeletal proteins resulting in changes in the viscoelastic properties of the host cell. PMID: 28226242
    4. While weak interactions between glycophorin and band 3 undoubtedly exist, glycophorin A and band 3 must have separate interactions in the membrane that control their lateral mobility. PMID: 27580023
    5. CD235a could be the most sensitive predictor similar to CD4. PMID: 25716234
    6. Glycophorin-A-rich microparticles are released from evolving growing thrombi into the distal perfusing blood, and can be measured in peripheral blood. CD235a(+) cMPs may constitute a novel systemic biomarker of ongoing thrombosis. PMID: 26239059
    7. The authors demonstrate that the initial vacuolar membrane around internalized Babesia divergens is formed from protein and lipid components of the red blood cells plasma membrane, including band 3, glycophorin A and spectrin. PMID: 25628009
    8. GPA-deficient band 3 null erythrocytes are resistant to malaria infection. PMID: 25778531
    9. MNSs blood group glycophorin variants in Taiwan: a genotype-serotype correlation study of 'Mi(a)' and St(a) with report of two new alleles for St(a). PMID: 24858913
    10. PfEBA-175 engages multiple glycans of GpA encoded by exon 3 and that the presentation of glycans is likely required for high-avidity binding. PMID: 25205096
    11. The expression of glycophorin A and osteoprotegerin is locally increased in carotid atherosclerotic lesions of symptomatic compared to asymptomatic patients. PMID: 23722820
    12. These data demonstrate the importance of PfEBA175 regions other than the DBL domains in the interaction with GYPA and merit their inclusion in an EBA175-based vaccine. PMID: 24043627
    13. GPA-NN mutagens contributed to the pathogenesis of chronic benzene poisoning. PMID: 17456399
    14. Beta-branched residues adjacent to GG4 motifs promote the efficient association of glycophorin A transmembrane helices. PMID: 21072853
    15. Data suggest that glycophorin A (Gpa) increases expression and activity of Cl-/HCO3- exchanger Ae1, that G719D mutation renders Ae1 mutant constructs GPA-unresponsive, and suggests a role for Ae1 amino acids 22-28 in GPA responsiveness. PMID: 21455273
    16. the GG genotype of the rs 1489759 HHIP single-nucleotide polymorphism (SNP) and the CC genotype of the rs 2202507 GYPA SNP confers a ''protective'' effect on COPD (OR 0.59, p50.006 for HHIP and OR50.65, p50.006 for GYPA) and lung cancer. PMID: 21119205
    17. Data indicate that changes of the glycophorin A dimerization propensities in different lipid bilayers suggest that the lipid bilayer thickness severely influences the monomer-dimer equilibrium of the transmembrane domain. PMID: 20603102
    18. ABH blood group antigens in N-glycan of human glycophorin A. PMID: 20434428
    19. The Sta gene found in this family has A246 & C252, C302, and G307. The putative crossing-over point is between nucleotides 252 to 302 in Intron 3. PMID: 20233359
    20. Energy transfer between differently labelled glycophorin A transmembrane helices decreased with increasing Sodium Dodecyl Sulfate mole fractions albeit without modifying the helicity of the peptides. PMID: 20074546
    21. Heterozygous missense mutation E758K in the human AE1/SLC4A1/band 3 gene in two unrelated patients with well-compensated hereditary spherostomatocytic anemia induces glycophorin A-independent, endogenous cation transport. PMID: 19907019
    22. Natural selection of polymorphisms/variants on the erythrocyte surface PMID: 11861881
    23. Somatic cell mutations found at the gene locus in erythrocytes of radiation workers at a nuclear facility PMID: 12492375
    24. Distinct regions of human glycophorin A enhance human red cell anion exchanger transport function and surface trafficking PMID: 12813056
    25. band 3 in the red cell can take up two different structures: one with high anion transport activity when glycophorin A is present and one with lower anion transport activity when glycophorin A is absent PMID: 14604989
    26. Porcine Kupffer cells bind xenogeneic human RBC by recognition of a carbohydrate epitope on glycophorin A. PMID: 16003235
    27. This study demonstrates the systematic classification of ten alleles (five M and five N alleles) of the MN blood group system to major or minor variations of the standard alleles. PMID: 16205834
    28. glycophorin A somatic mutation frequency levels in newborns are not related to maternal lifestyle factors or drug metabolizing enzyme genotypes PMID: 16424825
    29. GYPA & GYPB genotypes carrying MNSs antigens were analyzed in Thai patients with cerebral malaria & mild malaria; no statistical difference was found; it is concluded that MNSs antigens do not reveal the difference in susceptibility to cerebral malaria PMID: 17372674
    30. single mutations in glycophorin A can result in the observed fourth power relationship with age of oncet in polycythemia rubra vera PMID: 17452518
    31. GPA depletion does not alter band 3's native conformation at the DIDS binding site. It modulates a conformational equilibrium between 2 states of the binary complex formed by the competitive inhibitor DIDS, reversibly bound to properly folded band 3. PMID: 19071041
    32. AE1 and GPA form a complex in the endoplasmic reticulum of human K562 cells. PMID: 19438409

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  • 亞細胞定位：
    Cell membrane; Single-pass type I membrane protein. Note=Appears to be colocalized with SLC4A1.
  • 蛋白家族：
    Glycophorin A family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4702

    OMIM: 111300

    KEGG: hsa:2993

    STRING: 9606.ENSP00000354003

    UniGene: Hs.434973