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ITGB4 Recombinant Monoclonal Antibody

  • 中文名稱：
    ITGB4重組抗體
  • 貨號：
    CSB-RA011887MA1HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Overlay Peak curve showing A549 cells surface stained with CSB-RA011887MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    ITGB4 Recombinant Monoclonal Antibody（CSB-RA011887MA1HU）是一款高特異性抗體，靶向整合素β4（ITGB4）——一種參與細(xì)胞-基質(zhì)黏附和信號轉(zhuǎn)導(dǎo)的關(guān)鍵跨膜蛋白，在維持上皮細(xì)胞穩(wěn)態(tài)、組織修復(fù)及腫瘤微環(huán)境調(diào)控中發(fā)揮重要作用。本抗體采用重組技術(shù)制備，經(jīng)ELISA與流式細(xì)胞術(shù)（FC）嚴(yán)格驗(yàn)證，證實(shí)其可特異性識別天然構(gòu)象的ITGB4蛋白，適用于靶蛋白的定量檢測及細(xì)胞表面表達(dá)水平的精準(zhǔn)分析。在流式應(yīng)用中，推薦使用1:20-1:500稀釋范圍，可根據(jù)實(shí)驗(yàn)體系優(yōu)化調(diào)整。該產(chǎn)品通過嚴(yán)格的質(zhì)控流程確保批次間高一致性，適用于癌癥生物學(xué)研究中腫瘤細(xì)胞侵襲機(jī)制探索、上皮細(xì)胞遷移動態(tài)分析，以及組織再生相關(guān)基礎(chǔ)研究中的細(xì)胞黏附行為解析。其卓越的靈敏度與低交叉反應(yīng)性特點(diǎn)，為體外模型和原代細(xì)胞的靶蛋白功能研究提供可靠工具，尤其適用于需要精確定量或細(xì)胞群體分選的實(shí)驗(yàn)場景。
  • Uniprot No.：
  • 基因名：
  • 別名：
    CD 104 antibody; CD104 antibody; CD104 antigen antibody; gp150 antibody; Integrin beta 4 subunit antibody; Integrin beta-4 antibody; ITB4_HUMAN antibody; ITG B4 antibody; ITGB 4 antibody; Itgb4 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    Recombinant Human ITGB4 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5C2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    FC 1:20-1:500
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.
  • 基因功能參考文獻(xiàn)：
    1. High ITGB4 expression is associated with drug resistance in colorectal cancer. PMID: 30106452
    2. ITGB4 plays a tumorigenic and pro-metastatic role mediated by Slug PMID: 28084395
    3. correlation analysis indicated that the expression of ARRDC3 was negatively correlated with ITGbeta4 in clinical prostate cancer (PCa) tissues and cell lines. Our data revealed that ARRDC3 can serve as a tumor suppressor to inhibit PCa progression and an independent marker to predict the risk of biochemical recurrence and metastasis after radical resection of PCa. PMID: 28782483
    4. ITGB4(+) cancer stem cell (CSC)-enriched mesenchymal cells reside in an intermediate epithelial/mesenchymal phenotypic state. PMID: 28270621
    5. These findings suggest that the integrin beta4-FAK/Src signaling axis may play a crucial role in clonorchiasis-associated cholangiocarcinoma metastasis during tumor progression. PMID: 28286026
    6. MUC5AC interacts with integrin beta4 that mediates phosphorylation of FAK at Y397 leading to lung cancer cell migration. PMID: 26751774
    7. a significant reduction in the protein distribution of collagen IV (P<0.0001), collagen VII (P<0.001), collagen XVII (P<0.01), integrin beta4 (P<0.001) and laminin-332 (P<0.0001) in intrinsically aged skin. PMID: 27013376
    8. For the in vivo experiment, miR-182-5p overexpression also promoted the growth and progression of prostate cancer tumors. In this regard, we suggest that miR-182-5p may be a key androgen receptor-regulated factor that contributes to the development and metastasis of Chinese prostate cancers and may be a potential target for the early diagnosis and therapeutic studies of prostate cancer. PMID: 27109471
    9. data indicate that the beta4 integrin/FAK complex and subsequent FAK activation are essential regulators during the tumorigenicity of colon cancer PMID: 27178753
    10. This work investigates alpha6beta4 integrin genetic variations (A380T and R1281W) and breast cancer risk in an Argentinian population. No subjects carrying the R1281W mutation in the ITGB4 gene were found and this absence could reflect its high deleterious impact on proteins, so it would be eliminated from the population by natural selection. PMID: 27763564
    11. Herein, we present a female newborn with lethal Junctional epidermolysis bullosa with pyloric atresia caused by a novel beta4 integrin mutation. PMID: 27186702
    12. analysis of deletions in ITGB4 gene causing epidermolysis bullosa with pyloric atresia [case series] PMID: 26739954
    13. ITGB4 is overexpressed in hepatocellular carcinoma tissues and promotes metastases of HCC by conferring anchorage independence through EGFR-dependent FAK-AKT activation. PMID: 26996299
    14. High vimentin and low beta4 integrin protein levels are associated with poor survival in oral squamous carcinoma patients. PMID: 26646105
    15. Endothelial cell overexpression of mutant ITGB4 with specific tyrosines mutated to phenylalanine (Y1440, Y1526 Y1640, or Y1422) resulted in significantly attenuated CS-induced cytokine expression. PMID: 26572585
    16. This study describes a pro-metastatic EGFR/Src-dependent beta4 integrin/FAK complex that is involved in breast cancer malignancy and is a novel therapeutic target for triple-negative breast cancer. PMID: 26549523
    17. These data suggest that the p53(R248) mutant endows ovarian cancer cells with increased adhesiveness and that integrin beta4 and Akt signalling are associated with the mutation-enhanced ovarian cancer-mesothelial cell adhesion. PMID: 26223322
    18. Changes in beta-integrins gene expression in human prostate cancer cells following ITGB4 knockdown. PMID: 26395630
    19. PKD2 and RSK1 regulate integrin beta4 phosphorylation at threonine 1736 to stabilize keratinocyte cell adhesion and its hemidesmosomes. PMID: 26580203
    20. Data indicate that IGF binding protein-3 (IGFBP-3) reduced transcription of a variety of integrins, especially integrin beta4. PMID: 25945837
    21. Analysis showed that ITGB4 and VCL were upregulated in exosomes derived from taxane-resistant prostate cancer cells suggesting them as useful markers for progression of prostate cancer associated with taxane-resistance. PMID: 25997717
    22. Case Report: pyloric atresia-junctional epidermolysis bullosa syndrome with splicing abnormality of ITGB4 due to nucleotide substitutions far from splice site. PMID: 25728941
    23. ITGB4 stimulation leads to recruitment of 12-LOX from the cytosol to the membrane. PMID: 26037302
    24. The third and fourth fibronectin type III domains (FnIII-3,4) emerge as a structural and functional unit within the integrin beta 4. PMID: 25849406
    25. find that both Syk and LMP2A have an effect on ITGbeta4 cell surface expression PMID: 25531330
    26. Case Report: ITGB4 germline mutations in junctional epidermolysis bullosa without pylori atresia but profound genito-urinary involvement. PMID: 24807042
    27. Mutations in the 5' flanking region result in reduced integrin beta4 expression, which is related to increased risk of asthma. PMID: 24740264
    28. By a loss-of-function assay, we elucidate a novel feed-forward signaling pathway, integrin beta4 --> PI3K --> Akt --> FAK, by which TR3 mediates HUVEC migration. Furthermore, TR3/Nur77 regulates the expression of integrin beta4 by targeting its promoter activity. PMID: 25326539
    29. Data indicate that knockdown of integrin beta4 (ITGB4) reduced the migratory and invasive ability of pancreatic ductal adenocarcinoma cells. PMID: 25599535
    30. miR-21 is a key player in oncogenic EMT, its overexpression is controlled by the cooperation of genetic and epigenetic alterations, and its levels, along with ITGbeta4 and PDCD4 expression, could be exploited as a prognostic tool for CRC metastasis. PMID: 24149370
    31. underexpressed in circulating (4D culture) cells relative to 2D culture cells PMID: 25234746
    32. the diminishment of crosstalk between phosphorylated FAK and ITGB4 due to the down-regulation of Gli family transcription factors might play a pivotal role for inhibiting ovarian cancer progression PMID: 24533083
    33. Data indicate that sphingosine 1-phosphate (S1P) and hepatocyte growth factor (HGF) induced transloaction of integrin beta4, S1P receptors S1PR2 and S1PR3 to endothelial cell membrane caveolin-enriched microdomains (CEMs). PMID: 24851274
    34. Both annexin A7 and integrin beta4 were essential for small molecule, 6-amino-2, 3-dihydro-3-hydroxymethyl-1, 4-benzoxazine-induced autophagy. PMID: 24007983
    35. The expression of MPS-1 and ITGB4 is associated with poor outcomes in gastric cancer patients. PMID: 23803695
    36. beta4 and beta1 integrin protein and mRNA expression is elevated in PKP2-silenced keratinocytes. PMID: 23884246
    37. beta4 integrin rs8669 genotyping may help to identify a subgroup of HER-3-negative, K-RAS wild-type colorectal cancer patients who are more likely to benefit from anti-EGFR treatment. PMID: 23617461
    38. study reports two new non-Herlitz junctional epidermolysis bullosa cases carrying two novel ITGB4 mutations - c.3040C>T in exon 26 and c.4975G>T in exon 37 PMID: 23013259
    39. Post-translational regulation of beta4 by PTHrP (Parathyroid hormone-related protein) is mediated via attenuation of its proteolytic cleavage by caspases. PMID: 23499737
    40. Mechanistic studies indicated that mutant ITGB4 fails to promote transactivation of ErbB2 and c-Met in prostate tumor progenitor cells and cancer cell lines. PMID: 23348745
    41. S1PR1 and ITGB4 transactivation are rate-limiting events in the transduction of HGF signals via a dynamic c-Met complex resulting in enhanced EC barrier integrity PMID: 23212923
    42. Most actinic cheilitis cases showed reduced expression of integrin beta4 and superficially invasive squamous cell carcinoma lacked intergrin beta4 in the invasive front. PMID: 22917688
    43. invasive breast cancer cells confer an anoikis-resistant phenotype on myofibroblasts during tissue remodeling by inducing laminin-332 upregulation and integrin beta4 neoexpression PMID: 22673183
    44. MAPK activation is increased when [Ca(2+)](i) is increased, suggesting that calcineurin activates an additional mechanism that counteracts MAPK-induced beta4 phosphorylation. PMID: 22865863
    45. Silencing of ITGB4 in airway epithelial cells resulted in impaired antigen presentation processes and suppressed T cell proliferation. PMID: 22545078
    46. Mutational analysis disclosed compound heterozygosity for two novel nonsense mutations in the ITGB4 gene: c.600dupC/p.F201fsX14 and c.2533C>T/p.Q845X. PMID: 21969027
    47. the expression of integrin beta(4) is upregulated to sensitize the cells to low concentrations of netrin-4 for maintaining cell proliferation. PMID: 22496621
    48. beta4 can regulate SPARC expression and that SPARC is an effector of beta4-mediated invasion. PMID: 22308039
    49. AexU effector protein of Aeromonas veronii is a novel type three secretion system effector which specifically inactivates Rac1 to disrupt actin cytoskeleton and has an alternative cytotoxic pathway through beta4-integrin mediation. PMID: 21963679
    50. beta4 serine phosphorylation may have important role during squamous cell carcinoma invasion by destabilizing hemidesmosomes and facilitating migration. PMID: 21769085

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  • 相關(guān)疾病：
    Epidermolysis bullosa letalis, with pyloric atresia (EB-PA); Generalized atrophic benign epidermolysis bullosa (GABEB)
  • 亞細(xì)胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor. Cell junction, hemidesmosome. Note=Colocalizes with DST at the leading edge of migrating keratinocytes.
  • 蛋白家族：
    Integrin beta chain family
  • 組織特異性：
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6158

    OMIM: 147557

    KEGG: hsa:3691

    STRING: 9606.ENSP00000200181

    UniGene: Hs.632226