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MAS1L Recombinant Monoclonal Antibody

  • 中文名稱：
    MAS1L重組抗體
  • 貨號(hào)：
    CSB-RA971033A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue
      All lanes: MAS1L antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 43 kDa
      Observed band size: 36-50 kDa
    • Immunofluorescence staining of MCF-7 cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Immunofluorescence staining of Hela cell with CSB-RA971033A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 595-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA971033A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA971033A0HU是靶向MAS1L蛋白的重組單克隆抗體，適用于多種實(shí)驗(yàn)場(chǎng)景的科研需求。該抗體針對(duì)MAS1L受體特異性設(shè)計(jì)，可精確識(shí)別其天然構(gòu)象表位，在多種驗(yàn)證實(shí)驗(yàn)中展現(xiàn)出優(yōu)異的性能：Western Blot（WB）推薦稀釋比例1:500-1:2000，可清晰檢測(cè)目標(biāo)蛋白條帶；免疫熒光（IF）和流式細(xì)胞術(shù)（FC）分別在1:50-1:200稀釋范圍內(nèi)呈現(xiàn)高信噪比染色效果，適用于亞細(xì)胞定位及表面標(biāo)志物分析。MAS1L作為G蛋白偶聯(lián)受體家族成員，參與調(diào)控細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)和組織穩(wěn)態(tài)，本產(chǎn)品可支持其在細(xì)胞功能研究（如增殖調(diào)控、分化機(jī)制探索）及疾病模型（如心血管或腫瘤相關(guān)基礎(chǔ)研究）中的表達(dá)分析。經(jīng)ELISA驗(yàn)證，該抗體具備高批次穩(wěn)定性與低交叉反應(yīng)性，可滿足復(fù)雜樣本的定量檢測(cè)需求。適用于體外培養(yǎng)細(xì)胞、組織裂解液等樣本類型，為受體功能研究、信號(hào)通路解析及分子互作實(shí)驗(yàn)提供可靠工具。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Mas-related G-protein coupled receptor MRG (MAS-R) (MAS1-like), MAS1L, MRG
  • 反應(yīng)種屬：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human MAS1L
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    15B8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. Mas, MrgD, and MRG mediated Ang IV-stimulated AA release that was highest for Mas. While Ang III activated Mas and MrgX2, Ang II stimulated AA release via Mas and MRG. PMID: 18636314
  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Mas subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 13961

    OMIM: 607235

    KEGG: hsa:116511

    STRING: 9606.ENSP00000366331

    UniGene: Hs.533297