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  4. TUBB1 Recombinant Monoclonal Antibody

TUBB1 Recombinant Monoclonal Antibody

  • 中文名稱：
    TUBB1重組抗體
  • 貨號：
    CSB-RA867148MA1HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate
      All lanes: TUBB1 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 50 kDa
      Observed band size: 50 kDa
    • IHC image of CSB-RA867148MA1HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA867148MA1HU diluted at 1:300 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing NIH/3T3 cells stained with CSB-RA867148MA1HU (red line) at 1:200. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA867148MA1HU TUBB1重組單克隆抗體是針對β-微管蛋白家族成員TUBB1開發(fā)的高特異性檢測工具。該抗體靶向的TUBB1蛋白是細胞骨架的核心組成單元，在維持細胞形態(tài)、有絲分裂及細胞內(nèi)物質(zhì)運輸中發(fā)揮重要作用，其功能異常與巨核細胞成熟障礙及血小板生成異常密切相關(guān)。經(jīng)多種技術(shù)平臺驗證，本抗體在Western Blot中可清晰識別內(nèi)源性TUBB1蛋白，免疫組化實驗顯示其在細胞質(zhì)中的特異性定位，流式細胞分析進一步證實其對細胞表面微管結(jié)構(gòu)的識別能力。推薦的實驗優(yōu)化濃度范圍為：免疫印跡1:1000-5000，組織切片染色1:20-200，流式檢測1:20-200，適用于細胞骨架動態(tài)研究、血小板分化機制探索以及血液系統(tǒng)疾病模型的構(gòu)建。產(chǎn)品經(jīng)過嚴格的質(zhì)量控制，在不同實驗體系中均表現(xiàn)出優(yōu)異的批間一致性和信號穩(wěn)定性，為研究微管蛋白功能調(diào)控及相關(guān)病理機制提供可靠工具。
  • Uniprot No.：
  • 基因名：
  • 別名：
    2810484G07Rik antibody; beta 1 tubulin antibody; Beta tubulin 1, class VI antibody; Beta-tubulin antibody; Class VI beta tubulin antibody; dJ543J19.4 antibody; M(beta)1 antibody; TBB1_HUMAN antibody; TUBB1 antibody; Tubulin beta 1 class VI antibody; Tubulin beta-1 chain antibody; Tubulin, beta 1 antibody; tubulin, beta1 antibody
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human TUBB1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    20B1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    IHC 1:20-1:200
    FC 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 基因功能參考文獻：
    1. Neonatal platelets exhibit low levels of the Stx11-Munc18b complex (essential component of the SNARE machinery) and of beta1-tubulin. These developmental deficiencies are associated with defects in platelet adhesion, spreading and secretion. PMID: 29044293
    2. novel DCX mutation (p.D90G, NP_000546.2) appeared to be a major causative variant, whereas the novel mutation of TUBB1 (p.R62fsX, NP_110400.1) was found only in patients with more-severe intellectual disability in Familial pachygyria PMID: 26743950
    3. Analysis of the TUBB1 gene revealed three known missense variants in heterozygous state which in combination might explain the beta1-tubulin defect. PMID: 26540125
    4. TUBB1 R307H SNP is significantly associated with the degree of thrombocytopenia in congenital and acquired platelet disorders, and may affect platelets by altering microtubule behavior. PMID: 25529050
    5. Data indicate that ABCB1 protein, beta tubulin I and III (betaI, and betaIII tubulin) might contribute to the multidrug resistance (MDR) of MCF7/DOC and be potential therapeutic targets for overcoming MDR of breast cancer. PMID: 24894670
    6. TUBB1 mutation disrupting microtubule assembly impairs proplatelet formation and results in congenital macrothrombocytopenia. PMID: 24344610
    7. our findings define beta-tubulin VI as a hematologic isotype with significant genetic variation in humans that may affect the myelosuppresive action of microtubule-binding drugs PMID: 22805305
    8. homozygous status of P43 genetic polymorphism causes alterations in platelet ultrastructure PMID: 21384078
    9. Studies show that BFBTS bound and modified beta-tubulin at residue Cys12, forming beta-tubulin-SS-fluorobenzyl. PMID: 19996274
    10. SLPI localizes in part along the megakaaryocyte and platelet cytoskeleton by virtue of specific interactions with beta1 tubulin. PMID: 15315966
    11. the platelet Q43P beta1-tubulin substitution is frequent in the healthy population and may protect men against arterial thrombosis PMID: 15956286
    12. The TUBB1 Q43P polymorphism, by causing a lower reactivity in platelets carrying the variant form of b1-tubulin, protects against thrombotic disorders but increases the risk of intracerebral hemorrhage in men. PMID: 17488662
    13. biophysical analysis of carboxy-terminal tail conformation of human beta-tubulin isotypes PMID: 17993481
    14. Mutation of the beta1-tubulin gene associated with congenital macrothrombocytopenia affecting microtubule assembly. PMID: 18849486
    15. TUBB1 Q43P polymorphism does not protect against acute coronary syndrome and premature myocardial infarction. PMID: 19132255

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  • 相關(guān)疾?。?/div>
    Macrothrombocytopenia, autosomal dominant, TUBB1-related (MAD-TUBB1)
  • 亞細胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Tubulin family
  • 組織特異性：
    Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 16257

    OMIM: 612901

    KEGG: hsa:81027

    STRING: 9606.ENSP00000217133

    UniGene: Hs.303023