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  4. Acetyl-Histone H2A type 1-B/E (K9) Recombinant Monoclonal Antibody

Acetyl-Histone H2A type 1-B/E (K9) Recombinant Monoclonal Antibody

  • 中文名稱：
    乙酰-組蛋白 H2A type 1-B/E (K9)重組抗體
  • 貨號(hào)：
    CSB-RA010385A09acHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Immunocytochemistry analysis of CSB-RA010385A09acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010385A09acHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA010385A09acHU重組單克隆抗體特異性識(shí)別組蛋白H2A亞型1-B/E第9位賴氨酸乙?；揎棧ˋcetyl-Histone H2A type 1-B/E K9），該表觀遺傳標(biāo)記在染色質(zhì)重塑和基因轉(zhuǎn)錄調(diào)控中發(fā)揮重要作用。本抗體通過重組表達(dá)技術(shù)制備，具有高批次一致性和優(yōu)異特異性，經(jīng)ELISA、免疫細(xì)胞化學(xué)（ICC）及免疫熒光（IF）等多平臺(tái)驗(yàn)證，推薦使用濃度分別為ICC 1:50-1:500和IF 1:30-1:200。實(shí)驗(yàn)數(shù)據(jù)顯示其能精準(zhǔn)識(shí)別內(nèi)源性乙?；疕2A蛋白，適用于探究細(xì)胞分化、DNA損傷修復(fù)及表觀遺傳調(diào)控機(jī)制等研究領(lǐng)域。在染色質(zhì)動(dòng)態(tài)分析、干細(xì)胞重編程模型及表觀遺傳藥物篩選等科研場(chǎng)景中表現(xiàn)卓越，為研究組蛋白修飾與基因表達(dá)調(diào)控網(wǎng)絡(luò)提供可靠工具。
  • Uniprot No.：
  • 基因名：
    HIST1H2AB
  • 別名：
    H2a 615 antibody; H2A antibody; H2A GL101 antibody; H2A histone family member A antibody; H2A.1 antibody; H2A.2 antibody; H2A/a antibody; H2A/m antibody; H2A/O antibody; H2A/q antibody; H2A1B_HUMAN antibody; H2AFA antibody; H2AFE antibody; H2AFL antibody; H2AFM antibody; H2AFO antibody; H2AFQ antibody; HIST1H2AE antibody; HIST1H2AJ antibody; HIST2H2AA antibody; HIST2H2AA3 antibody; HIST2H2AB antibody; HIST2H2AC antibody; Histone 1 H2ae antibody; Histone 2 H2aa3 antibody; Histone 2 H2ab antibody; Histone 2 H2ac antibody; Histone H2A type 1 B antibody; Histone H2A type 1 C antibody; Histone H2A type 1 E antibody; Histone H2A type 1 J antibody; Histone H2A type 1-B/E antibody; Histone H2A.2 antibody; Histone H2A/a antibody; Histone H2A/m antibody; MGC74460 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    3H5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, ICC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    ICC 1:50-1:500
    IF 1:30-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 亞細(xì)胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2A family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 4734

    OMIM: 602786

    KEGG: hsa:3012

    STRING: 9606.ENSP00000303373

    UniGene: Hs.121017