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  4. Recombinant Drosophila melanogaster DNA polymerase subunit gamma-1, mitochondrial (tam), partial

Recombinant Drosophila melanogaster DNA polymerase subunit gamma-1, mitochondrial (tam), partial

  • 中文名稱：
    黑腹果蠅tam重組蛋白
  • 貨號(hào)：
    CSB-YP630644DLU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅tam重組蛋白
  • 貨號(hào)：
    CSB-EP630644DLU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅tam重組蛋白
  • 貨號(hào)：
    CSB-EP630644DLU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅tam重組蛋白
  • 貨號(hào)：
    CSB-BP630644DLU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅tam重組蛋白
  • 貨號(hào)：
    CSB-MP630644DLU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tam
  • Uniprot No.：
  • 別名：
    tam; DNApol-gamma; DNApol-gamma125; DNApolG1; l(2)34Dc; l(2)br5; MtPolA; pol; gamma-alpha; Polg; CG8987DNA polymerase subunit gamma-1; mitochondrial; EC 2.7.7.7; 3'-5' exodeoxyribonuclease; EC 3.1.11.-; Mitochondrial DNA polymerase catalytic subunit; Protein tamas
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    As the catalytic component of the DNA polymerase gamma complex is involved in the replication of mitochondrial DNA (mtDNA). Has both 5'-3' DNA polymerase and a highly mispair-specific 3'-5' exonuclease activity. At the end of mtDNA replication DNA ends are ligated to produce a closed circular mtDNA molecule, its exonuclease activity is required for formation of these ligatable ends by preventing DNA synthesis from continuing past the 5'-end of downstream DNA into duplex DNA regions. Does not possess DNA primase activity, does not catalyze strand displacement synthesis and does not contain a 5'-3' exonuclease activity to catalyze nick translation. Important for promoting the elimination of paternal mitochondrial DNA during spermatogenesis, however its exact role in this function has not yet been identified and appears to be independent of its 3'-5'-exonuclease activity and only partially dependent on its DNA polymerase activity.
  • 基因功能參考文獻(xiàn)：
    1. Knockdown of the nuclear-encoded mtDNA polymerase, Tamas, produces a more complete block of mtDNA elimination. PMID: 28318978
    2. Muscle-specific overexpression of the catalytic subunit of DNA polymerase gamma induces pupal lethality in Drosophila melanogaster. PMID: 23729397
    3. DNA-binding mutants of mitochondrial single-stranded DNA-binding protein are defective in stimulation of DNA synthesis by pol gamma PMID: 14754882
  • 亞細(xì)胞定位：
    Mitochondrion.
  • 蛋白家族：
    DNA polymerase type-A family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dme:Dmel_CG8987

    STRING: 7227.FBpp0080148

    UniGene: Dm.2292