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  4. Recombinant Drosophila melanogaster Protein enabled (ena), partial

Recombinant Drosophila melanogaster Protein enabled (ena), partial

  • 中文名稱：
    黑腹果蠅ena重組蛋白
  • 貨號(hào)：
    CSB-YP851514DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅ena重組蛋白
  • 貨號(hào)：
    CSB-EP851514DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅ena重組蛋白
  • 貨號(hào)：
    CSB-EP851514DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅ena重組蛋白
  • 貨號(hào)：
    CSB-BP851514DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅ena重組蛋白
  • 貨號(hào)：
    CSB-MP851514DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ena
  • Uniprot No.：
  • 別名：
    ena; enb; CG15112; Protein enabled
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Functions, together with Abl, trio and fra, in a complex signaling network that regulates axon guidance at the CNS midline. Required in part for robo-mediated repulsive axon guidance. May be involved in lamellipodial dynamics.
  • 基因功能參考文獻(xiàn)：
    1. The Abl tyrosine kinase signaling network controls cell migration, epithelial organization, axon patterning and other aspects of development. Abl suppresses the actin-regulatory factor Enabled, and we find that Abl also acts through the GEF Trio to stimulate the signaling activity of Rac GTPase. PMID: 28087633
    2. Abl/Ena signaling controls the subcellular architecture of the Golgi complex in Drosophila photoreceptor neurons through its regulation of the Golgi-associated actin cytoskeleton. PMID: 25103244
    3. the activities of Ena/VASP and the WAVE regulatory complex are intimately linked to ensure optimal control of actin polymerization during cell migration and development PMID: 25203209
    4. Ena is both sufficient and necessary to account for miR-8-mediated regulation of subsynaptic reticulum architecture. PMID: 24718988
    5. Ena regulates force balance in the somatic gonadal precursors by sequestering DE-cad, leading to the morphogenetic movement required for gonad compaction. PMID: 23300733
    6. Canoe (Cno) and Pyd are required for proper Ena localization during dorsal closure, and strong genetic interactions suggest that Cno, Pyd, and Ena act together in regulating or anchoring the actin cytoskeleton during dorsal closure PMID: 21508316
    7. Ena localises to regions of actin dynamics within migrating haemocytes, stimulates lamellipodial dynamics and positively regulates the number and length of filopodia. PMID: 21045209
    8. In abl mutants, Enabled accumulates ectopically at the apical cortex where excess actin is observed, suggesting that Abl regulates Ena's subcellular localization. PMID: 14676307
    9. Ena/VASP, when not inhibited by the Abl pathway, can bind kinesin heavy chain and reduce its transport activity in axons PMID: 15975902
    10. Enabled (Ena), a substrate for Abl, promotes the formation of both dendritic branches and actin-rich spine-like protrusions of dendritic arborization neurons, an effect opposite to that of Abl PMID: 16003769
    11. Ena modulates filopodial number and length, thus influencing the speed of epithelial zippering and the ability of cells to match with correct neighbors in embryonic morphogenesis. PMID: 17507404
    12. Drosophila enabled/vasodilator stimulated phosphoprotein (Ena/VASP) proteins are candidates for driving cytoskeleton modulation under the membrane. PMID: 18378777
    13. Enabled and Capping actin regulator proteins shape oogenesis. PMID: 19576200

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  • 亞細(xì)胞定位：
    Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Note=Expressed at the leading edge of lamellipodia. Colocalizes with chic at the periphery of cells.
  • 蛋白家族：
    Ena/VASP family
  • 組織特異性：
    Expressed in axons of the embryonic nervous system.
  • 數(shù)據(jù)庫鏈接：