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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anoxybacillus flavithermus Glutamine amidotransferase subunit pdxT (pdxT) CSB-YP467360BZO
CSB-EP467360BZO
CSB-BP467360BZO
CSB-MP467360BZO
CSB-EP467360BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP467361BZO
CSB-EP467361BZO
CSB-BP467361BZO
CSB-MP467361BZO
CSB-EP467361BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP467362BZO
CSB-EP467362BZO
CSB-BP467362BZO
CSB-MP467362BZO
CSB-EP467362BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP467363BZO
CSB-EP467363BZO
CSB-BP467363BZO
CSB-MP467363BZO
CSB-EP467363BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S2 (rpsB) CSB-YP467364BZO
CSB-EP467364BZO
CSB-BP467364BZO
CSB-MP467364BZO
CSB-EP467364BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Ribosome biogenesis GTPase A (rbgA) CSB-YP467365BZO
CSB-EP467365BZO
CSB-BP467365BZO
CSB-MP467365BZO
CSB-EP467365BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Phosphopantetheine adenylyltransferase (coaD) CSB-YP467366BZO
CSB-EP467366BZO
CSB-BP467366BZO
CSB-MP467366BZO
CSB-EP467366BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus UPF0756 membrane protein Aflv_0503 (Aflv_0503), partial CSB-YP467367BZO1
CSB-EP467367BZO1
CSB-BP467367BZO1
CSB-MP467367BZO1
CSB-EP467367BZO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Ribulokinase (araB), partial CSB-YP467368BZO
CSB-EP467368BZO
CSB-BP467368BZO
CSB-MP467368BZO
CSB-EP467368BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP467369BZO
CSB-EP467369BZO
CSB-BP467369BZO
CSB-MP467369BZO
CSB-EP467369BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Valine--tRNA ligase (valS), partial CSB-YP467370BZO
CSB-EP467370BZO
CSB-BP467370BZO
CSB-MP467370BZO
CSB-EP467370BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 3-dehydroquinate dehydratase (aroQ) CSB-YP467371BZO
CSB-EP467371BZO
CSB-BP467371BZO
CSB-MP467371BZO
CSB-EP467371BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA) CSB-YP467372BZO
CSB-EP467372BZO
CSB-BP467372BZO
CSB-MP467372BZO
CSB-EP467372BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus CCA-adding enzyme (cca) CSB-YP467373BZO
CSB-EP467373BZO
CSB-BP467373BZO
CSB-MP467373BZO
CSB-EP467373BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Small, acid-soluble spore protein O (sspO) CSB-YP467374BZO
CSB-EP467374BZO
CSB-BP467374BZO
CSB-MP467374BZO
CSB-EP467374BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Protein hfq (hfq) CSB-YP467375BZO
CSB-EP467375BZO
CSB-BP467375BZO
CSB-MP467375BZO
CSB-EP467375BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S19 (rpsS) CSB-YP467376BZO
CSB-EP467376BZO
CSB-BP467376BZO
CSB-MP467376BZO
CSB-EP467376BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 50S ribosomal protein L14 (rplN) CSB-YP467377BZO
CSB-EP467377BZO
CSB-BP467377BZO
CSB-MP467377BZO
CSB-EP467377BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus 30S ribosomal protein S5 (rpsE) CSB-YP467378BZO
CSB-EP467378BZO
CSB-BP467378BZO
CSB-MP467378BZO
CSB-EP467378BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anoxybacillus flavithermus Undecaprenyl-diphosphatase (uppP), partial CSB-YP467379BZO
CSB-EP467379BZO
CSB-BP467379BZO
CSB-MP467379BZO
CSB-EP467379BZO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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