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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O17:K52:H18 Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP486287EOG
CSB-EP486287EOG
CSB-BP486287EOG
CSB-MP486287EOG
CSB-EP486287EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP486288EOG
CSB-EP486288EOG
CSB-BP486288EOG
CSB-MP486288EOG
CSB-EP486288EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Aspartate--tRNA ligase (aspS) CSB-YP486289EOG
CSB-EP486289EOG
CSB-BP486289EOG
CSB-MP486289EOG
CSB-EP486289EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 D-cysteine desulfhydrase (dcyD) CSB-YP486290EOG
CSB-EP486290EOG
CSB-BP486290EOG
CSB-MP486290EOG
CSB-EP486290EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Protein mtfA (mtfA) CSB-YP486291EOG
CSB-EP486291EOG
CSB-BP486291EOG
CSB-MP486291EOG
CSB-EP486291EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Histidinol-phosphate aminotransferase (hisC) CSB-YP486292EOG
CSB-EP486292EOG
CSB-BP486292EOG
CSB-MP486292EOG
CSB-EP486292EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Methionine--tRNA ligase (metG) CSB-YP486293EOG
CSB-EP486293EOG
CSB-BP486293EOG
CSB-MP486293EOG
CSB-EP486293EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) CSB-YP486294EOG
CSB-EP486294EOG
CSB-BP486294EOG
CSB-MP486294EOG
CSB-EP486294EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP486295EOG1
CSB-EP486295EOG1
CSB-BP486295EOG1
CSB-MP486295EOG1
CSB-EP486295EOG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Threonine/serine transporter TdcC (tdcC), partial CSB-YP486296EOG
CSB-EP486296EOG
CSB-BP486296EOG
CSB-MP486296EOG
CSB-EP486296EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 DnaA initiator-associating protein diaA (diaA) CSB-YP486297EOG
CSB-EP486297EOG
CSB-BP486297EOG
CSB-MP486297EOG
CSB-EP486297EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 50S ribosomal protein L27 (rpmA) CSB-YP486298EOG
CSB-EP486298EOG
CSB-BP486298EOG
CSB-MP486298EOG
CSB-EP486298EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP486299EOG
CSB-EP486299EOG
CSB-BP486299EOG
CSB-MP486299EOG
CSB-EP486299EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP486300EOG1
CSB-EP486300EOG1
CSB-BP486300EOG1
CSB-MP486300EOG1
CSB-EP486300EOG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 30S ribosomal protein S8 (rpsH) CSB-YP486301EOG
CSB-EP486301EOG
CSB-BP486301EOG
CSB-MP486301EOG
CSB-EP486301EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 30S ribosomal protein S17 (rpsQ) CSB-YP486302EOG
CSB-EP486302EOG
CSB-BP486302EOG
CSB-MP486302EOG
CSB-EP486302EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 50S ribosomal protein L3 (rplC) CSB-YP486303EOG
CSB-EP486303EOG
CSB-BP486303EOG
CSB-MP486303EOG
CSB-EP486303EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Sulfurtransferase TusD (tusD) CSB-YP486304EOG
CSB-EP486304EOG
CSB-BP486304EOG
CSB-MP486304EOG
CSB-EP486304EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Protein tsgA (tsgA), partial CSB-YP486305EOG
CSB-EP486305EOG
CSB-BP486305EOG
CSB-MP486305EOG
CSB-EP486305EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Fe/S biogenesis protein NfuA (nfuA) CSB-YP486306EOG
CSB-EP486306EOG
CSB-BP486306EOG
CSB-MP486306EOG
CSB-EP486306EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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