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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O17:K52:H18 Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP486307EOG
CSB-EP486307EOG
CSB-BP486307EOG
CSB-MP486307EOG
CSB-EP486307EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP486308EOG
CSB-EP486308EOG
CSB-BP486308EOG
CSB-MP486308EOG
CSB-EP486308EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP486309EOG
CSB-EP486309EOG
CSB-BP486309EOG
CSB-MP486309EOG
CSB-EP486309EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Protein-export protein secB (secB) CSB-YP486310EOG
CSB-EP486310EOG
CSB-BP486310EOG
CSB-MP486310EOG
CSB-EP486310EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 50S ribosomal protein L28 (rpmB) CSB-YP486311EOG
CSB-EP486311EOG
CSB-BP486311EOG
CSB-MP486311EOG
CSB-EP486311EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Adenine deaminase (ade) CSB-YP486312EOG
CSB-EP486312EOG
CSB-BP486312EOG
CSB-MP486312EOG
CSB-EP486312EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 50S ribosomal protein L34 (rpmH) CSB-YP486313EOG
CSB-EP486313EOG
CSB-BP486313EOG
CSB-MP486313EOG
CSB-EP486313EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Multidrug resistance protein MdtL (mdtL), partial CSB-YP486314EOG
CSB-EP486314EOG
CSB-BP486314EOG
CSB-MP486314EOG
CSB-EP486314EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 ATPase ravA (ravA) CSB-YP486315EOG
CSB-EP486315EOG
CSB-BP486315EOG
CSB-MP486315EOG
CSB-EP486315EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 ATP-dependent RNA helicase rhlB (rhlB) CSB-YP486316EOG
CSB-EP486316EOG
CSB-BP486316EOG
CSB-MP486316EOG
CSB-EP486316EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Tyrosine recombinase XerC (xerC) CSB-YP486317EOG
CSB-EP486317EOG
CSB-BP486317EOG
CSB-MP486317EOG
CSB-EP486317EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 D-tyrosyl-tRNA (Tyr) deacylase CSB-YP486318EOG
CSB-EP486318EOG
CSB-BP486318EOG
CSB-MP486318EOG
CSB-EP486318EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Rhamnulokinase (rhaB) CSB-YP486319EOG
CSB-EP486319EOG
CSB-BP486319EOG
CSB-MP486319EOG
CSB-EP486319EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP486320EOG
CSB-EP486320EOG
CSB-BP486320EOG
CSB-MP486320EOG
CSB-EP486320EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 50S ribosomal protein L1 (rplA) CSB-YP486321EOG
CSB-EP486321EOG
CSB-BP486321EOG
CSB-MP486321EOG
CSB-EP486321EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 NADH pyrophosphatase (nudC) CSB-YP486322EOG
CSB-EP486322EOG
CSB-BP486322EOG
CSB-MP486322EOG
CSB-EP486322EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Cytochrome c-552 (nrfA) CSB-YP486323EOG
CSB-EP486323EOG
CSB-BP486323EOG
CSB-MP486323EOG
CSB-EP486323EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 Uncharacterized protein YjeA (yjeA) CSB-YP486324EOG
CSB-EP486324EOG
CSB-BP486324EOG
CSB-MP486324EOG
CSB-EP486324EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 HTH-type transcriptional repressor NsrR (nsrR) CSB-YP486325EOG
CSB-EP486325EOG
CSB-BP486325EOG
CSB-MP486325EOG
CSB-EP486325EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O17:K52:H18 30S ribosomal protein S6 (rpsF) CSB-YP486326EOG
CSB-EP486326EOG
CSB-BP486326EOG
CSB-MP486326EOG
CSB-EP486326EOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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