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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Clostridium cellulolyticum 50S ribosomal protein L20 (rplT) CSB-YP493328DUL
CSB-EP493328DUL
CSB-BP493328DUL
CSB-MP493328DUL
CSB-EP493328DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP493329DUL
CSB-EP493329DUL
CSB-BP493329DUL
CSB-MP493329DUL
CSB-EP493329DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Ketol-acid reductoisomerase (ilvC) CSB-YP493330DUL
CSB-EP493330DUL
CSB-BP493330DUL
CSB-MP493330DUL
CSB-EP493330DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP493331DUL
CSB-EP493331DUL
CSB-BP493331DUL
CSB-MP493331DUL
CSB-EP493331DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Protein hfq (hfq) CSB-YP493332DUL
CSB-EP493332DUL
CSB-BP493332DUL
CSB-MP493332DUL
CSB-EP493332DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP493333DUL
CSB-EP493333DUL
CSB-BP493333DUL
CSB-MP493333DUL
CSB-EP493333DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP493334DUL
CSB-EP493334DUL
CSB-BP493334DUL
CSB-MP493334DUL
CSB-EP493334DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L28 (rpmB) CSB-YP493335DUL
CSB-EP493335DUL
CSB-BP493335DUL
CSB-MP493335DUL
CSB-EP493335DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP493336DUL
CSB-EP493336DUL
CSB-BP493336DUL
CSB-MP493336DUL
CSB-EP493336DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L9 (rplI) CSB-YP493337DUL
CSB-EP493337DUL
CSB-BP493337DUL
CSB-MP493337DUL
CSB-EP493337DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Probable transcriptional regulatory protein Ccel_0181 (Ccel_0181) CSB-YP493338DUL
CSB-EP493338DUL
CSB-BP493338DUL
CSB-MP493338DUL
CSB-EP493338DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Putative sporulation transcription regulator WhiA (whiA) CSB-YP493339DUL
CSB-EP493339DUL
CSB-BP493339DUL
CSB-MP493339DUL
CSB-EP493339DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L11 (rplK) CSB-YP493340DUL
CSB-EP493340DUL
CSB-BP493340DUL
CSB-MP493340DUL
CSB-EP493340DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP493341DUL
CSB-EP493341DUL
CSB-BP493341DUL
CSB-MP493341DUL
CSB-EP493341DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP493342DUL
CSB-EP493342DUL
CSB-BP493342DUL
CSB-MP493342DUL
CSB-EP493342DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Probable septum site-determining protein MinC (minC) CSB-YP493343DUL
CSB-EP493343DUL
CSB-BP493343DUL
CSB-MP493343DUL
CSB-EP493343DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Ribosome maturation factor RimP (rimP) CSB-YP493344DUL
CSB-EP493344DUL
CSB-BP493344DUL
CSB-MP493344DUL
CSB-EP493344DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Cell division protein SepF (sepF) CSB-YP493345DUL
CSB-EP493345DUL
CSB-BP493345DUL
CSB-MP493345DUL
CSB-EP493345DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum UPF0251 protein Ccel_0627 (Ccel_0627) CSB-YP493346DUL
CSB-EP493346DUL
CSB-BP493346DUL
CSB-MP493346DUL
CSB-EP493346DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum 50S ribosomal protein L23 (rplW) CSB-YP493347DUL
CSB-EP493347DUL
CSB-BP493347DUL
CSB-MP493347DUL
CSB-EP493347DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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