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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Anaeromyxobacter dehalogenans Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP493668BYS
CSB-EP493668BYS
CSB-BP493668BYS
CSB-MP493668BYS
CSB-EP493668BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP493669BYS
CSB-EP493669BYS
CSB-BP493669BYS
CSB-MP493669BYS
CSB-EP493669BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans DNA repair protein recO (recO) CSB-YP493670BYS
CSB-EP493670BYS
CSB-BP493670BYS
CSB-MP493670BYS
CSB-EP493670BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans Membrane protein insertase YidC (yidC), partial CSB-YP493671BYS1
CSB-EP493671BYS1
CSB-BP493671BYS1
CSB-MP493671BYS1
CSB-EP493671BYS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP493672BYS
CSB-EP493672BYS
CSB-BP493672BYS
CSB-MP493672BYS
CSB-EP493672BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans V-type ATP synthase subunit D (atpD) CSB-YP493673BYS
CSB-EP493673BYS
CSB-BP493673BYS
CSB-MP493673BYS
CSB-EP493673BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP493674BYS
CSB-EP493674BYS
CSB-BP493674BYS
CSB-MP493674BYS
CSB-EP493674BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans Transcriptional repressor NrdR (nrdR) CSB-YP493675BYS
CSB-EP493675BYS
CSB-BP493675BYS
CSB-MP493675BYS
CSB-EP493675BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans 30S ribosomal protein S15 (rpsO) CSB-YP493676BYS
CSB-EP493676BYS
CSB-BP493676BYS
CSB-MP493676BYS
CSB-EP493676BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anaeromyxobacter dehalogenans Thiazole synthase (thiG) CSB-YP493677BYS
CSB-EP493677BYS
CSB-BP493677BYS
CSB-MP493677BYS
CSB-EP493677BYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Talaromyces stipitatus Methylthioribulose-1-phosphate dehydratase (mde1) CSB-YP493678TKZ
CSB-EP493678TKZ
CSB-BP493678TKZ
CSB-MP493678TKZ
CSB-EP493678TKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Talaromyces stipitatus Mitochondrial distribution and morphology protein 12 (mdm12) CSB-YP493679TKZ
CSB-EP493679TKZ
CSB-BP493679TKZ
CSB-MP493679TKZ
CSB-EP493679TKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Glutamyl-tRNA reductase (hemA) CSB-YP493680DJN
CSB-EP493680DJN
CSB-BP493680DJN
CSB-MP493680DJN
CSB-EP493680DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP493681DJN
CSB-EP493681DJN
CSB-BP493681DJN
CSB-MP493681DJN
CSB-EP493681DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP493682DJN
CSB-EP493682DJN
CSB-BP493682DJN
CSB-MP493682DJN
CSB-EP493682DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans UPF0296 protein Ddes_1405 (Ddes_1405) CSB-YP493683DJN
CSB-EP493683DJN
CSB-BP493683DJN
CSB-MP493683DJN
CSB-EP493683DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans 50S ribosomal protein L11 (rplK) CSB-YP493684DJN
CSB-EP493684DJN
CSB-BP493684DJN
CSB-MP493684DJN
CSB-EP493684DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP493685DJN1
CSB-EP493685DJN1
CSB-BP493685DJN1
CSB-MP493685DJN1
CSB-EP493685DJN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP493686DJN
CSB-EP493686DJN
CSB-BP493686DJN
CSB-MP493686DJN
CSB-EP493686DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio desulfuricans Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP493687DJN
CSB-EP493687DJN
CSB-BP493687DJN
CSB-MP493687DJN
CSB-EP493687DJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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