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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus carnosus Ribosomal protein L11 methyltransferase (prmA) CSB-YP494428FLK
CSB-EP494428FLK
CSB-BP494428FLK
CSB-MP494428FLK
CSB-EP494428FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Elongation factor 4 (lepA), partial CSB-YP494429FLK
CSB-EP494429FLK
CSB-BP494429FLK
CSB-MP494429FLK
CSB-EP494429FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Probable endonuclease 4 (nfo) CSB-YP494430FLK
CSB-EP494430FLK
CSB-BP494430FLK
CSB-MP494430FLK
CSB-EP494430FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP494431FLK
CSB-EP494431FLK
CSB-BP494431FLK
CSB-MP494431FLK
CSB-EP494431FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Peptide methionine sulfoxide reductase MsrB (msrB) CSB-YP494432FLK
CSB-EP494432FLK
CSB-BP494432FLK
CSB-MP494432FLK
CSB-EP494432FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP494433FLK
CSB-EP494433FLK
CSB-BP494433FLK
CSB-MP494433FLK
CSB-EP494433FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO CSB-YP494434FLK
CSB-EP494434FLK
CSB-BP494434FLK
CSB-MP494434FLK
CSB-EP494434FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP494435FLK
CSB-EP494435FLK
CSB-BP494435FLK
CSB-MP494435FLK
CSB-EP494435FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus UPF0348 protein Sca_0752 (Sca_0752) CSB-YP494436FLK
CSB-EP494436FLK
CSB-BP494436FLK
CSB-MP494436FLK
CSB-EP494436FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus UPF0356 protein Sca_0714 (Sca_0714) CSB-YP494437FLK
CSB-EP494437FLK
CSB-BP494437FLK
CSB-MP494437FLK
CSB-EP494437FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Bifunctional purine biosynthesis protein PurH (purH) CSB-YP494438FLK
CSB-EP494438FLK
CSB-BP494438FLK
CSB-MP494438FLK
CSB-EP494438FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis Bifunctional protein GlmU (glmU) CSB-YP494439FNR
CSB-EP494439FNR
CSB-BP494439FNR
CSB-MP494439FNR
CSB-EP494439FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis Fructose-1,6-bisphosphatase class 3 (fbp), partial CSB-YP494440FNR
CSB-EP494440FNR
CSB-BP494440FNR
CSB-MP494440FNR
CSB-EP494440FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis DNA ligase (ligA), partial CSB-YP494441FNR
CSB-EP494441FNR
CSB-BP494441FNR
CSB-MP494441FNR
CSB-EP494441FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP494442FNR
CSB-EP494442FNR
CSB-BP494442FNR
CSB-MP494442FNR
CSB-EP494442FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis Homoserine kinase (thrB) CSB-YP494443FNR
CSB-EP494443FNR
CSB-BP494443FNR
CSB-MP494443FNR
CSB-EP494443FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis Deoxyribose-phosphate aldolase (deoC) CSB-YP494444FNR
CSB-EP494444FNR
CSB-BP494444FNR
CSB-MP494444FNR
CSB-EP494444FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP494445FNR
CSB-EP494445FNR
CSB-BP494445FNR
CSB-MP494445FNR
CSB-EP494445FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis Mannonate dehydratase (uxuA) CSB-YP494446FNR
CSB-EP494446FNR
CSB-BP494446FNR
CSB-MP494446FNR
CSB-EP494446FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus uberis Phosphate acyltransferase (plsX) CSB-YP494447FNR
CSB-EP494447FNR
CSB-BP494447FNR
CSB-MP494447FNR
CSB-EP494447FNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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