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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus carnosus 50S ribosomal protein L36 (rpmJ) CSB-YP494788FLK
CSB-EP494788FLK
CSB-BP494788FLK
CSB-MP494788FLK
CSB-EP494788FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus 50S ribosomal protein L31 type B (rpmE2) CSB-YP494789FLK
CSB-EP494789FLK
CSB-BP494789FLK
CSB-MP494789FLK
CSB-EP494789FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Uracil phosphoribosyltransferase (upp) CSB-YP494790FLK
CSB-EP494790FLK
CSB-BP494790FLK
CSB-MP494790FLK
CSB-EP494790FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus 2-isopropylmalate synthase (leuA), partial CSB-YP494791FLK
CSB-EP494791FLK
CSB-BP494791FLK
CSB-MP494791FLK
CSB-EP494791FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Diacylglycerol kinase (dagK) CSB-YP494792FLK
CSB-EP494792FLK
CSB-BP494792FLK
CSB-MP494792FLK
CSB-EP494792FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus tRNA (guanine-N (7)-)-methyltransferase CSB-YP494793FLK
CSB-EP494793FLK
CSB-BP494793FLK
CSB-MP494793FLK
CSB-EP494793FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus UPF0173 metal-dependent hydrolase Sca_1312 (Sca_1312) CSB-YP494794FLK
CSB-EP494794FLK
CSB-BP494794FLK
CSB-MP494794FLK
CSB-EP494794FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) CSB-YP494795FLK
CSB-EP494795FLK
CSB-BP494795FLK
CSB-MP494795FLK
CSB-EP494795FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP494796FLK
CSB-EP494796FLK
CSB-BP494796FLK
CSB-MP494796FLK
CSB-EP494796FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Shikimate dehydrogenase (aroE) CSB-YP494797FLK
CSB-EP494797FLK
CSB-BP494797FLK
CSB-MP494797FLK
CSB-EP494797FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Probable rRNA maturation factor (Sca_1192) CSB-YP494798FLK
CSB-EP494798FLK
CSB-BP494798FLK
CSB-MP494798FLK
CSB-EP494798FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Arginine repressor (argR) CSB-YP494799FLK
CSB-EP494799FLK
CSB-BP494799FLK
CSB-MP494799FLK
CSB-EP494799FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus 3-dehydroquinate synthase (aroB) CSB-YP494800FLK
CSB-EP494800FLK
CSB-BP494800FLK
CSB-MP494800FLK
CSB-EP494800FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Dihydrodipicolinate synthase (dapA) CSB-YP494801FLK
CSB-EP494801FLK
CSB-BP494801FLK
CSB-MP494801FLK
CSB-EP494801FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP494802FLK1
CSB-EP494802FLK1
CSB-BP494802FLK1
CSB-MP494802FLK1
CSB-EP494802FLK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Ribosome-binding factor A (rbfA) CSB-YP494803FLK
CSB-EP494803FLK
CSB-BP494803FLK
CSB-MP494803FLK
CSB-EP494803FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Tyrosine recombinase XerC (xerC) CSB-YP494804FLK
CSB-EP494804FLK
CSB-BP494804FLK
CSB-MP494804FLK
CSB-EP494804FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus UPF0122 protein Sca_0859 (Sca_0859) CSB-YP494805FLK
CSB-EP494805FLK
CSB-BP494805FLK
CSB-MP494805FLK
CSB-EP494805FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP494806FLK
CSB-EP494806FLK
CSB-BP494806FLK
CSB-MP494806FLK
CSB-EP494806FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP494807FLK
CSB-EP494807FLK
CSB-BP494807FLK
CSB-MP494807FLK
CSB-EP494807FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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