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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus anthracis Septation ring formation regulator EzrA (ezrA), partial CSB-YP503590BQF
CSB-EP503590BQF
CSB-BP503590BQF
CSB-MP503590BQF
CSB-EP503590BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 30S ribosomal protein S4 (rpsD) CSB-YP503591BQF
CSB-EP503591BQF
CSB-BP503591BQF
CSB-MP503591BQF
CSB-EP503591BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Large-conductance mechanosensitive channel (mscL), partial CSB-YP503592BQF1
CSB-EP503592BQF1
CSB-BP503592BQF1
CSB-MP503592BQF1
CSB-EP503592BQF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP503593BQF
CSB-EP503593BQF
CSB-BP503593BQF
CSB-MP503593BQF
CSB-EP503593BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis tRNA (guanine-N (7)-)-methyltransferase CSB-YP503594BQF
CSB-EP503594BQF
CSB-BP503594BQF
CSB-MP503594BQF
CSB-EP503594BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP503595BQF
CSB-EP503595BQF
CSB-BP503595BQF
CSB-MP503595BQF
CSB-EP503595BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Leucine--tRNA ligase (leuS), partial CSB-YP503596BQF
CSB-EP503596BQF
CSB-BP503596BQF
CSB-MP503596BQF
CSB-EP503596BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP503597BQF
CSB-EP503597BQF
CSB-BP503597BQF
CSB-MP503597BQF
CSB-EP503597BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylformylglycinamidine synthase 1 (purQ) CSB-YP503598BQF
CSB-EP503598BQF
CSB-BP503598BQF
CSB-MP503598BQF
CSB-EP503598BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP503599BQF
CSB-EP503599BQF
CSB-BP503599BQF
CSB-MP503599BQF
CSB-EP503599BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP503600BQF
CSB-EP503600BQF
CSB-BP503600BQF
CSB-MP503600BQF
CSB-EP503600BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP503601BQF
CSB-EP503601BQF
CSB-BP503601BQF
CSB-MP503601BQF
CSB-EP503601BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Heptaprenylglyceryl phosphate synthase (pcrB) CSB-YP503602BQF
CSB-EP503602BQF
CSB-BP503602BQF
CSB-MP503602BQF
CSB-EP503602BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis DNA ligase (ligA), partial CSB-YP503603BQF
CSB-EP503603BQF
CSB-BP503603BQF
CSB-MP503603BQF
CSB-EP503603BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial CSB-YP503604BQF
CSB-EP503604BQF
CSB-BP503604BQF
CSB-MP503604BQF
CSB-EP503604BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP503605BQF
CSB-EP503605BQF
CSB-BP503605BQF
CSB-MP503605BQF
CSB-EP503605BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP503606BQF
CSB-EP503606BQF
CSB-BP503606BQF
CSB-MP503606BQF
CSB-EP503606BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP503607BQF
CSB-EP503607BQF
CSB-BP503607BQF
CSB-MP503607BQF
CSB-EP503607BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Hydroxyethylthiazole kinase (thiM) CSB-YP503608BQF
CSB-EP503608BQF
CSB-BP503608BQF
CSB-MP503608BQF
CSB-EP503608BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus anthracis Thiamine-phosphate synthase (thiE) CSB-YP503609BQF
CSB-EP503609BQF
CSB-BP503609BQF
CSB-MP503609BQF
CSB-EP503609BQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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