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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella melitensis biotype 2 Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP505870BWT
CSB-EP505870BWT
CSB-BP505870BWT
CSB-MP505870BWT
CSB-EP505870BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP505871BWT
CSB-EP505871BWT
CSB-BP505871BWT
CSB-MP505871BWT
CSB-EP505871BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 tRNA (guanine-N (1)-)-methyltransferase CSB-YP505872BWT
CSB-EP505872BWT
CSB-BP505872BWT
CSB-MP505872BWT
CSB-EP505872BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Probable intracellular septation protein A (BMEA_A1992), partial CSB-YP505873BWT1
CSB-EP505873BWT1
CSB-BP505873BWT1
CSB-MP505873BWT1
CSB-EP505873BWT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L28 (rpmB) CSB-YP505874BWT
CSB-EP505874BWT
CSB-BP505874BWT
CSB-MP505874BWT
CSB-EP505874BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Putative phosphotransferase BMEA_A2129 (BMEA_A2129) CSB-YP505875BWT
CSB-EP505875BWT
CSB-BP505875BWT
CSB-MP505875BWT
CSB-EP505875BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP505876BWT
CSB-EP505876BWT
CSB-BP505876BWT
CSB-MP505876BWT
CSB-EP505876BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L35 (rpmI) CSB-YP505877BWT
CSB-EP505877BWT
CSB-BP505877BWT
CSB-MP505877BWT
CSB-EP505877BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Probable rRNA maturation factor (BMEA_A2215) CSB-YP505878BWT
CSB-EP505878BWT
CSB-BP505878BWT
CSB-MP505878BWT
CSB-EP505878BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP505879BWT
CSB-EP505879BWT
CSB-BP505879BWT
CSB-MP505879BWT
CSB-EP505879BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP505880BWT
CSB-EP505880BWT
CSB-BP505880BWT
CSB-MP505880BWT
CSB-EP505880BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Lipoprotein signal peptidase (lspA), partial CSB-YP505881BWT1
CSB-EP505881BWT1
CSB-BP505881BWT1
CSB-MP505881BWT1
CSB-EP505881BWT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UDP-N-acetylglucosamine 1-carboxyvinyltransferase (murA) CSB-YP505882BWT
CSB-EP505882BWT
CSB-BP505882BWT
CSB-MP505882BWT
CSB-EP505882BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP505883BWT
CSB-EP505883BWT
CSB-BP505883BWT
CSB-MP505883BWT
CSB-EP505883BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L9 (rplI) CSB-YP505884BWT
CSB-EP505884BWT
CSB-BP505884BWT
CSB-MP505884BWT
CSB-EP505884BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Cytochrome c oxidase assembly protein CtaG (ctaG), partial CSB-YP505885BWT
CSB-EP505885BWT
CSB-BP505885BWT
CSB-MP505885BWT
CSB-EP505885BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 SsrA-binding protein (smpB) CSB-YP505886BWT
CSB-EP505886BWT
CSB-BP505886BWT
CSB-MP505886BWT
CSB-EP505886BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP505887BWT
CSB-EP505887BWT
CSB-BP505887BWT
CSB-MP505887BWT
CSB-EP505887BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Transcriptional repressor NrdR (nrdR) CSB-YP505888BWT
CSB-EP505888BWT
CSB-BP505888BWT
CSB-MP505888BWT
CSB-EP505888BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP505889BWT
CSB-EP505889BWT
CSB-BP505889BWT
CSB-MP505889BWT
CSB-EP505889BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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