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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brevibacillus brevis Xanthine phosphoribosyltransferase (xpt) CSB-YP507350BWL
CSB-EP507350BWL
CSB-BP507350BWL
CSB-MP507350BWL
CSB-EP507350BWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brevibacillus brevis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP507351BWL
CSB-EP507351BWL
CSB-BP507351BWL
CSB-MP507351BWL
CSB-EP507351BWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Lysylphosphatidylglycerol biosynthesis bifunctional protein LysX (lysX), partial CSB-YP507352RLK
CSB-EP507352RLK
CSB-BP507352RLK
CSB-MP507352RLK
CSB-EP507352RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Mycothiol acetyltransferase (mshD) CSB-YP507353RLK
CSB-EP507353RLK
CSB-BP507353RLK
CSB-MP507353RLK
CSB-EP507353RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP507354RLK
CSB-EP507354RLK
CSB-BP507354RLK
CSB-MP507354RLK
CSB-EP507354RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP507355RLK
CSB-EP507355RLK
CSB-BP507355RLK
CSB-MP507355RLK
CSB-EP507355RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Chaperone protein DnaK (dnaK), partial CSB-YP507356RLK
CSB-EP507356RLK
CSB-BP507356RLK
CSB-MP507356RLK
CSB-EP507356RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Glutamyl-tRNA reductase (hemA) CSB-YP507357RLK
CSB-EP507357RLK
CSB-BP507357RLK
CSB-MP507357RLK
CSB-EP507357RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L10 (rplJ) CSB-YP507358RLK
CSB-EP507358RLK
CSB-BP507358RLK
CSB-MP507358RLK
CSB-EP507358RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 30S ribosomal protein S12 (rpsL) CSB-YP507359RLK
CSB-EP507359RLK
CSB-BP507359RLK
CSB-MP507359RLK
CSB-EP507359RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L2 (rplB) CSB-YP507360RLK
CSB-EP507360RLK
CSB-BP507360RLK
CSB-MP507360RLK
CSB-EP507360RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 50S ribosomal protein L14 (rplN) CSB-YP507361RLK
CSB-EP507361RLK
CSB-BP507361RLK
CSB-MP507361RLK
CSB-EP507361RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Adenylate kinase (adk) CSB-YP507362RLK
CSB-EP507362RLK
CSB-BP507362RLK
CSB-MP507362RLK
CSB-EP507362RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 30S ribosomal protein S9 (rpsI) CSB-YP507363RLK
CSB-EP507363RLK
CSB-BP507363RLK
CSB-MP507363RLK
CSB-EP507363RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis UPF0182 protein RER_22310 (RER_22310), partial CSB-YP507364RLK
CSB-EP507364RLK
CSB-BP507364RLK
CSB-MP507364RLK
CSB-EP507364RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP507365RLK
CSB-EP507365RLK
CSB-BP507365RLK
CSB-MP507365RLK
CSB-EP507365RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Phosphopantetheine adenylyltransferase (coaD) CSB-YP507366RLK
CSB-EP507366RLK
CSB-BP507366RLK
CSB-MP507366RLK
CSB-EP507366RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis 30S ribosomal protein S15 (rpsO) CSB-YP507367RLK
CSB-EP507367RLK
CSB-BP507367RLK
CSB-MP507367RLK
CSB-EP507367RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Transcriptional repressor NrdR (nrdR) CSB-YP507368RLK
CSB-EP507368RLK
CSB-BP507368RLK
CSB-MP507368RLK
CSB-EP507368RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Probable transcriptional regulatory protein RER_29220 (RER_29220) CSB-YP507369RLK
CSB-EP507369RLK
CSB-BP507369RLK
CSB-MP507369RLK
CSB-EP507369RLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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