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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella melitensis biotype 2 ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP508690BWT
CSB-EP508690BWT
CSB-BP508690BWT
CSB-MP508690BWT
CSB-EP508690BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Indole-3-glycerol phosphate synthase (trpC) CSB-YP508691BWT
CSB-EP508691BWT
CSB-BP508691BWT
CSB-MP508691BWT
CSB-EP508691BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP508692BWT
CSB-EP508692BWT
CSB-BP508692BWT
CSB-MP508692BWT
CSB-EP508692BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Protein RecA (recA) CSB-YP508693BWT
CSB-EP508693BWT
CSB-BP508693BWT
CSB-MP508693BWT
CSB-EP508693BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L6 (rplF) CSB-YP508694BWT
CSB-EP508694BWT
CSB-BP508694BWT
CSB-MP508694BWT
CSB-EP508694BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 30S ribosomal protein S17 (rpsQ) CSB-YP508695BWT
CSB-EP508695BWT
CSB-BP508695BWT
CSB-MP508695BWT
CSB-EP508695BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L4 (rplD) CSB-YP508696BWT
CSB-EP508696BWT
CSB-BP508696BWT
CSB-MP508696BWT
CSB-EP508696BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 50S ribosomal protein L7/L12 (rplL) CSB-YP508697BWT
CSB-EP508697BWT
CSB-BP508697BWT
CSB-MP508697BWT
CSB-EP508697BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UPF0758 protein BMEA_A1330 (BMEA_A1330) CSB-YP508698BWT
CSB-EP508698BWT
CSB-BP508698BWT
CSB-MP508698BWT
CSB-EP508698BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Flagellar L-ring protein (flgH) CSB-YP508699BWT
CSB-EP508699BWT
CSB-BP508699BWT
CSB-MP508699BWT
CSB-EP508699BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 DNA mismatch repair protein mutL (mutL), partial CSB-YP508700BWT
CSB-EP508700BWT
CSB-BP508700BWT
CSB-MP508700BWT
CSB-EP508700BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP508701BWT
CSB-EP508701BWT
CSB-BP508701BWT
CSB-MP508701BWT
CSB-EP508701BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP508702BWT
CSB-EP508702BWT
CSB-BP508702BWT
CSB-MP508702BWT
CSB-EP508702BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 UPF0309 protein BMEA_B0892 (BMEA_B0892) CSB-YP508703BWT
CSB-EP508703BWT
CSB-BP508703BWT
CSB-MP508703BWT
CSB-EP508703BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 Acetylglutamate kinase (argB) CSB-YP508704BWT
CSB-EP508704BWT
CSB-BP508704BWT
CSB-MP508704BWT
CSB-EP508704BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 2 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP508705BWT
CSB-EP508705BWT
CSB-BP508705BWT
CSB-MP508705BWT
CSB-EP508705BWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dasypus novemcinctus Cyclin-dependent kinase 14 (CDK14) CSB-YP508706DIO
CSB-EP508706DIO
CSB-BP508706DIO
CSB-MP508706DIO
CSB-EP508706DIO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Signal peptidase complex catalytic subunit SEC11 (SEC11), partial CSB-YP508707ERP
CSB-EP508707ERP
CSB-BP508707ERP
CSB-MP508707ERP
CSB-EP508707ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Cyanate hydratase (CYN1) CSB-YP508708ERP
CSB-EP508708ERP
CSB-BP508708ERP
CSB-MP508708ERP
CSB-EP508708ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccidioides brasiliensis Mitochondrial zinc maintenance protein 1, mitochondrial (MZM1), partial CSB-YP508709ERP
CSB-EP508709ERP
CSB-BP508709ERP
CSB-MP508709ERP
CSB-EP508709ERP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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