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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arthroderma otae Fe-S cluster assembly protein DRE2 (DRE2) CSB-YP510271DOP
CSB-EP510271DOP
CSB-BP510271DOP
CSB-MP510271DOP
CSB-EP510271DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Subtilisin-like protease 9 (SUB9) CSB-YP510272DOP
CSB-EP510272DOP
CSB-BP510272DOP
CSB-MP510272DOP
CSB-EP510272DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae 40S ribosomal protein S1 (RPS1) CSB-YP510273DOP
CSB-EP510273DOP
CSB-BP510273DOP
CSB-MP510273DOP
CSB-EP510273DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Required for respiratory growth protein 9, mitochondrial (RRG9) CSB-YP510274DOP
CSB-EP510274DOP
CSB-BP510274DOP
CSB-MP510274DOP
CSB-EP510274DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Signal peptidase complex catalytic subunit SEC11 (SEC11), partial CSB-YP510275DOP
CSB-EP510275DOP
CSB-BP510275DOP
CSB-MP510275DOP
CSB-EP510275DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Tripeptidyl-peptidase SED4 (SED4) CSB-YP510276DOP
CSB-EP510276DOP
CSB-BP510276DOP
CSB-MP510276DOP
CSB-EP510276DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable D-xylulose reductase A (xdhA) CSB-YP510277DOP
CSB-EP510277DOP
CSB-BP510277DOP
CSB-MP510277DOP
CSB-EP510277DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Carboxypeptidase S1 homolog A (SCPA), partial CSB-YP510278DOP
CSB-EP510278DOP
CSB-BP510278DOP
CSB-MP510278DOP
CSB-EP510278DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Subtilisin-like protease 10 (SUB10) CSB-YP510279DOP
CSB-EP510279DOP
CSB-BP510279DOP
CSB-MP510279DOP
CSB-EP510279DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Probable dipeptidyl-aminopeptidase B (DAPB), partial CSB-YP510280DOP
CSB-EP510280DOP
CSB-BP510280DOP
CSB-MP510280DOP
CSB-EP510280DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthroderma otae Subtilisin-like protease 2 (SUB2) CSB-YP510281DOP
CSB-EP510281DOP
CSB-BP510281DOP
CSB-MP510281DOP
CSB-EP510281DOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Pentafunctional AROM polypeptide (BDCG_01028), partial CSB-YP510282AZD
CSB-EP510282AZD
CSB-BP510282AZD
CSB-MP510282AZD
CSB-EP510282AZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Pheromone-processing carboxypeptidase KEX1 (KEX1), partial CSB-YP510283AZD
CSB-EP510283AZD
CSB-BP510283AZD
CSB-MP510283AZD
CSB-EP510283AZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Transcription activator of gluconeogenesis BDCG_02812 (BDCG_02812), partial CSB-YP510284AZD
CSB-EP510284AZD
CSB-BP510284AZD
CSB-MP510284AZD
CSB-EP510284AZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis High osmolarity signaling protein SHO1 (SHO1), partial CSB-YP510285AZD1
CSB-EP510285AZD1
CSB-BP510285AZD1
CSB-MP510285AZD1
CSB-EP510285AZD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Leucine aminopeptidase 1 (LAP1) CSB-YP510286AZD
CSB-EP510286AZD
CSB-BP510286AZD
CSB-MP510286AZD
CSB-EP510286AZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (BDCG_09234), partial CSB-YP510287AZD
CSB-EP510287AZD
CSB-BP510287AZD
CSB-MP510287AZD
CSB-EP510287AZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma conjunctivae Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP510288MWF
CSB-EP510288MWF
CSB-BP510288MWF
CSB-MP510288MWF
CSB-EP510288MWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opisthacanthus cayaporum Insecticidal toxin OcyC10 CSB-YP510289OEM
CSB-EP510289OEM
CSB-BP510289OEM
CSB-MP510289OEM
CSB-EP510289OEM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ajellomyces dermatitidis Nuclear distribution protein PAC1 (PAC1) CSB-YP510290AZE
CSB-EP510290AZE
CSB-BP510290AZE
CSB-MP510290AZE
CSB-EP510290AZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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