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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Probable xanthine dehydrogenase subunit D (pucD), partial CSB-YP518356BRJ
CSB-EP518356BRJ
CSB-BP518356BRJ
CSB-MP518356BRJ
CSB-EP518356BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Extracellular ribonuclease (bsn) CSB-YP518357BRJ
CSB-EP518357BRJ
CSB-BP518357BRJ
CSB-MP518357BRJ
CSB-EP518357BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable ABC transporter permease protein yurM (yurM), partial CSB-YP518358BRJ1
CSB-EP518358BRJ1
CSB-BP518358BRJ1
CSB-MP518358BRJ1
CSB-EP518358BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase YurR (yurR) CSB-YP518359BRJ
CSB-EP518359BRJ
CSB-BP518359BRJ
CSB-MP518359BRJ
CSB-EP518359BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis NifU-like protein (nifU) CSB-YP518360BRJ
CSB-EP518360BRJ
CSB-BP518360BRJ
CSB-MP518360BRJ
CSB-EP518360BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methionine import system permease protein MetP (metP), partial CSB-YP518361BRJ1
CSB-EP518361BRJ1
CSB-BP518361BRJ1
CSB-MP518361BRJ1
CSB-EP518361BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yusF (yusF) CSB-YP518362BRJ
CSB-EP518362BRJ
CSB-BP518362BRJ
CSB-MP518362BRJ
CSB-EP518362BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable 3-hydroxyacyl-CoA dehydrogenase (fadN), partial CSB-YP518363BRJ
CSB-EP518363BRJ
CSB-BP518363BRJ
CSB-MP518363BRJ
CSB-EP518363BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yusP (yusP), partial CSB-YP518364BRJ
CSB-EP518364BRJ
CSB-BP518364BRJ
CSB-MP518364BRJ
CSB-EP518364BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yusU (yusU) CSB-YP518365BRJ
CSB-EP518365BRJ
CSB-BP518365BRJ
CSB-MP518365BRJ
CSB-EP518365BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Transcriptional regulatory protein liaR (liaR) CSB-YP518366BRJ
CSB-EP518366BRJ
CSB-BP518366BRJ
CSB-MP518366BRJ
CSB-EP518366BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein liaI (liaI), partial CSB-YP518367BRJ1
CSB-EP518367BRJ1
CSB-BP518367BRJ1
CSB-MP518367BRJ1
CSB-EP518367BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvgK (yvgK) CSB-YP518368BRJ
CSB-EP518368BRJ
CSB-BP518368BRJ
CSB-MP518368BRJ
CSB-EP518368BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP518369BRJ
CSB-EP518369BRJ
CSB-BP518369BRJ
CSB-MP518369BRJ
CSB-EP518369BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Disulfide bond formation protein D (bdbD) CSB-YP518370BRJ
CSB-EP518370BRJ
CSB-BP518370BRJ
CSB-MP518370BRJ
CSB-EP518370BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Copper chaperone CopZ (copZ) CSB-YP518371BRJ
CSB-EP518371BRJ
CSB-BP518371BRJ
CSB-MP518371BRJ
CSB-EP518371BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase YvaA (yvaA) CSB-YP518372BRJ
CSB-EP518372BRJ
CSB-BP518372BRJ
CSB-MP518372BRJ
CSB-EP518372BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yvaF (yvaF) CSB-YP518373BRJ
CSB-EP518373BRJ
CSB-BP518373BRJ
CSB-MP518373BRJ
CSB-EP518373BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable protein-export membrane protein SecG (secG), partial CSB-YP518374BRJ1
CSB-EP518374BRJ1
CSB-BP518374BRJ1
CSB-MP518374BRJ1
CSB-EP518374BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator yvaP (yvaP) CSB-YP518375BRJ
CSB-EP518375BRJ
CSB-BP518375BRJ
CSB-MP518375BRJ
CSB-EP518375BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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