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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana GDSL esterase/lipase At2g23540 (At2g23540) CSB-YP525690DOA
CSB-EP525690DOA
CSB-BP525690DOA
CSB-MP525690DOA
CSB-EP525690DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nicotianamine synthase 3 (NAS3) CSB-YP525691DOA
CSB-EP525691DOA
CSB-BP525691DOA
CSB-MP525691DOA
CSB-EP525691DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative casein kinase II subunit beta-4 (At2g44680) CSB-YP525692DOA
CSB-EP525692DOA
CSB-BP525692DOA
CSB-MP525692DOA
CSB-EP525692DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GDSL esterase/lipase At1g09390 (At1g09390) CSB-YP525693DOA
CSB-EP525693DOA
CSB-BP525693DOA
CSB-MP525693DOA
CSB-EP525693DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable thiol methyltransferase 1 (HOL2) CSB-YP525694DOA
CSB-EP525694DOA
CSB-BP525694DOA
CSB-MP525694DOA
CSB-EP525694DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dihydrodipicolinate reductase 1, chloroplastic (DAPB1) CSB-YP525695DOA
CSB-EP525695DOA
CSB-BP525695DOA
CSB-MP525695DOA
CSB-EP525695DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At2g44130 (At2g44130) CSB-YP525696DOA
CSB-EP525696DOA
CSB-BP525696DOA
CSB-MP525696DOA
CSB-EP525696DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At2g02890 (At2g02890), partial CSB-YP525697DOA
CSB-EP525697DOA
CSB-BP525697DOA
CSB-MP525697DOA
CSB-EP525697DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable membrane-associated kinase regulator 4 (MAKR4) CSB-YP525698DOA
CSB-EP525698DOA
CSB-BP525698DOA
CSB-MP525698DOA
CSB-EP525698DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Gibberellin-regulated protein 8 (At2g39540) CSB-YP525699DOA
CSB-EP525699DOA
CSB-BP525699DOA
CSB-MP525699DOA
CSB-EP525699DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutathione S-transferase TCHQD (TCHQD) CSB-YP525700DOA
CSB-EP525700DOA
CSB-BP525700DOA
CSB-MP525700DOA
CSB-EP525700DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable S-acyltransferase At2g40990 (At2g40990), partial CSB-YP525701DOA1
CSB-EP525701DOA1
CSB-BP525701DOA1
CSB-MP525701DOA1
CSB-EP525701DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter B family member 4 (ABCB4), partial CSB-YP525702DOA
CSB-EP525702DOA
CSB-BP525702DOA
CSB-MP525702DOA
CSB-EP525702DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana U-box domain-containing protein 19 (PUB19), partial CSB-YP525703DOA
CSB-EP525703DOA
CSB-BP525703DOA
CSB-MP525703DOA
CSB-EP525703DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Membrane-associated protein VIPP1, chloroplastic (VIPP1) CSB-YP525704DOA
CSB-EP525704DOA
CSB-BP525704DOA
CSB-MP525704DOA
CSB-EP525704DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Leucine-rich repeat receptor-like protein CLAVATA2 (CLV2), partial CSB-YP525705DOA
CSB-EP525705DOA
CSB-BP525705DOA
CSB-MP525705DOA
CSB-EP525705DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UPF0187 protein At2g45870, chloroplastic (At2g45870), partial CSB-YP525706DOA
CSB-EP525706DOA
CSB-BP525706DOA
CSB-MP525706DOA
CSB-EP525706DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL33 (ATL33), partial CSB-YP525708DOA1
CSB-EP525708DOA1
CSB-BP525708DOA1
CSB-MP525708DOA1
CSB-EP525708DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter G family member 1 (ABCG1), partial CSB-YP525709DOA1
CSB-EP525709DOA1
CSB-BP525709DOA1
CSB-MP525709DOA1
CSB-EP525709DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Serine/threonine-protein kinase-like protein CCR2 (CCR2), partial CSB-YP525710DOA
CSB-EP525710DOA
CSB-BP525710DOA
CSB-MP525710DOA
CSB-EP525710DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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