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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Glycine max Cytochrome P450 71D10 (CYP71D10), partial CSB-YP526175GGV
CSB-EP526175GGV
CSB-BP526175GGV
CSB-MP526175GGV
CSB-EP526175GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Ethylene receptor (ETR1), partial CSB-YP526176NHE1
CSB-EP526176NHE1
CSB-BP526176NHE1
CSB-MP526176NHE1
CSB-EP526176NHE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable 3-beta-hydroxysteroid-Delta (8),Delta (7)-isomerase, partial CSB-YP526177DOA1
CSB-EP526177DOA1
CSB-BP526177DOA1
CSB-MP526177DOA1
CSB-EP526177DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Arginase (AG1) CSB-YP526178GGV
CSB-EP526178GGV
CSB-BP526178GGV
CSB-MP526178GGV
CSB-EP526178GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gossypium hirsutum Probable phospholipid hydroperoxide glutathione peroxidase CSB-YP526179GHB
CSB-EP526179GHB
CSB-BP526179GHB
CSB-MP526179GHB
CSB-EP526179GHB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fritillaria agrestis Cytochrome b6-f complex iron-sulfur subunit, chloroplastic (petC), partial CSB-YP526180FFL1
CSB-EP526180FFL1
CSB-BP526180FFL1
CSB-MP526180FFL1
CSB-EP526180FFL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nucleoside diphosphate kinase III, chloroplastic/mitochondrial (NDPK3) CSB-YP526181DOA
CSB-EP526181DOA
CSB-BP526181DOA
CSB-MP526181DOA
CSB-EP526181DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Carboxyvinyl-carboxyphosphonate phosphorylmutase, chloroplastic (At1g77060) CSB-YP526182DOA
CSB-EP526182DOA
CSB-BP526182DOA
CSB-MP526182DOA
CSB-EP526182DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative flavin-containing monooxygenase YUCCA7 (YUC7) CSB-YP526183DOA
CSB-EP526183DOA
CSB-BP526183DOA
CSB-MP526183DOA
CSB-EP526183DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nitrile-specifier protein 2 (NSP2) CSB-YP526184DOA
CSB-EP526184DOA
CSB-BP526184DOA
CSB-MP526184DOA
CSB-EP526184DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Hexaprenyldihydroxybenzoate methyltransferase, mitochondrial (COQ3) CSB-YP526185DOA
CSB-EP526185DOA
CSB-BP526185DOA
CSB-MP526185DOA
CSB-EP526185DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g18840 (PCMP-E101), partial CSB-YP526186DOA
CSB-EP526186DOA
CSB-BP526186DOA
CSB-MP526186DOA
CSB-EP526186DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Allantoate deiminase, chloroplastic (AAH) CSB-YP526187DOA
CSB-EP526187DOA
CSB-BP526187DOA
CSB-MP526187DOA
CSB-EP526187DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana SKP1-like protein 11 (ASK11) CSB-YP526188DOA
CSB-EP526188DOA
CSB-BP526188DOA
CSB-MP526188DOA
CSB-EP526188DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF091 (ERF091) CSB-YP526189DOA
CSB-EP526189DOA
CSB-BP526189DOA
CSB-MP526189DOA
CSB-EP526189DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At4g21240 (At4g21240) CSB-YP526190DOA
CSB-EP526190DOA
CSB-BP526190DOA
CSB-MP526190DOA
CSB-EP526190DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At4g22180 (At4g22180) CSB-YP526191DOA
CSB-EP526191DOA
CSB-BP526191DOA
CSB-MP526191DOA
CSB-EP526191DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger A20 and AN1 domain-containing stress-associated protein 9 (SAP9) CSB-YP526192DOA
CSB-EP526192DOA
CSB-BP526192DOA
CSB-MP526192DOA
CSB-EP526192DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Aluminum-activated malate transporter 12 (ALMT12), partial CSB-YP526193DOA1
CSB-EP526193DOA1
CSB-BP526193DOA1
CSB-MP526193DOA1
CSB-EP526193DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g21880, mitochondrial (At4g21880), partial CSB-YP526194DOA
CSB-EP526194DOA
CSB-BP526194DOA
CSB-MP526194DOA
CSB-EP526194DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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