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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella suis 7-cyano-7-deazaguanine synthase (queC) CSB-YP532049BWU
CSB-EP532049BWU
CSB-BP532049BWU
CSB-MP532049BWU
CSB-EP532049BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP532050BWU
CSB-EP532050BWU
CSB-BP532050BWU
CSB-MP532050BWU
CSB-EP532050BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis tRNA modification GTPase MnmE (mnmE) CSB-YP532051BWU
CSB-EP532051BWU
CSB-BP532051BWU
CSB-MP532051BWU
CSB-EP532051BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis UPF0262 protein BSUIS_A0274 (BSUIS_A0274) CSB-YP532052BWU
CSB-EP532052BWU
CSB-BP532052BWU
CSB-MP532052BWU
CSB-EP532052BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Protein ApaG (apaG) CSB-YP532053BWU
CSB-EP532053BWU
CSB-BP532053BWU
CSB-MP532053BWU
CSB-EP532053BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis GTPase Der (der) CSB-YP532054BWU
CSB-EP532054BWU
CSB-BP532054BWU
CSB-MP532054BWU
CSB-EP532054BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP532055BWU
CSB-EP532055BWU
CSB-BP532055BWU
CSB-MP532055BWU
CSB-EP532055BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis 50S ribosomal protein L9 (rplI) CSB-YP532056BWU
CSB-EP532056BWU
CSB-BP532056BWU
CSB-MP532056BWU
CSB-EP532056BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Porin omp2b (omp2b) CSB-YP532057BWU
CSB-EP532057BWU
CSB-BP532057BWU
CSB-MP532057BWU
CSB-EP532057BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis N utilization substance protein B homolog (nusB) CSB-YP532058BWU
CSB-EP532058BWU
CSB-BP532058BWU
CSB-MP532058BWU
CSB-EP532058BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP532059BWU
CSB-EP532059BWU
CSB-BP532059BWU
CSB-MP532059BWU
CSB-EP532059BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP532060BWU
CSB-EP532060BWU
CSB-BP532060BWU
CSB-MP532060BWU
CSB-EP532060BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP532061BWU
CSB-EP532061BWU
CSB-BP532061BWU
CSB-MP532061BWU
CSB-EP532061BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Arginine--tRNA ligase (argS), partial CSB-YP532062BWU
CSB-EP532062BWU
CSB-BP532062BWU
CSB-MP532062BWU
CSB-EP532062BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP532063BWU
CSB-EP532063BWU
CSB-BP532063BWU
CSB-MP532063BWU
CSB-EP532063BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laccaria bicolor Translation factor GUF1, mitochondrial (GUF1), partial CSB-YP532064LLZ
CSB-EP532064LLZ
CSB-BP532064LLZ
CSB-MP532064LLZ
CSB-EP532064LLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laccaria bicolor GDP-mannose transporter (VRG4), partial CSB-YP532065LLZ
CSB-EP532065LLZ
CSB-BP532065LLZ
CSB-MP532065LLZ
CSB-EP532065LLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Laccaria bicolor Type 1 phosphatases regulator YPI1 (YPI1) CSB-YP532066LLZ
CSB-EP532066LLZ
CSB-BP532066LLZ
CSB-MP532066LLZ
CSB-EP532066LLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Golgin candidate 6 (GC6), partial CSB-YP532067DOA
CSB-EP532067DOA
CSB-BP532067DOA
CSB-MP532067DOA
CSB-EP532067DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus durissus terrificus Thrombin-like enzyme gyroxin B1.3 CSB-YP532068DYD
CSB-EP532068DYD
CSB-BP532068DYD
CSB-MP532068DYD
CSB-EP532068DYD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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