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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clavibacter michiganensis subsp. sepedonicus DNA-directed RNA polymerase subunit alpha (rpoA) CSB-YP532269DTV
CSB-EP532269DTV
CSB-BP532269DTV
CSB-MP532269DTV
CSB-EP532269DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP532270DTV
CSB-EP532270DTV
CSB-BP532270DTV
CSB-MP532270DTV
CSB-EP532270DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP532271DTV
CSB-EP532271DTV
CSB-BP532271DTV
CSB-MP532271DTV
CSB-EP532271DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus 2-isopropylmalate synthase (leuA), partial CSB-YP532272DTV
CSB-EP532272DTV
CSB-BP532272DTV
CSB-MP532272DTV
CSB-EP532272DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus 50S ribosomal protein L28 (rpmB) CSB-YP532273DTV
CSB-EP532273DTV
CSB-BP532273DTV
CSB-MP532273DTV
CSB-EP532273DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Ribosome-binding factor A (rbfA) CSB-YP532274DTV
CSB-EP532274DTV
CSB-BP532274DTV
CSB-MP532274DTV
CSB-EP532274DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Anthranilate phosphoribosyltransferase (trpD) CSB-YP532275DTV
CSB-EP532275DTV
CSB-BP532275DTV
CSB-MP532275DTV
CSB-EP532275DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Alanine--tRNA ligase (alaS) CSB-YP532276DTV
CSB-EP532276DTV
CSB-BP532276DTV
CSB-MP532276DTV
CSB-EP532276DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Ribosome maturation factor RimM (rimM) CSB-YP532277DTV
CSB-EP532277DTV
CSB-BP532277DTV
CSB-MP532277DTV
CSB-EP532277DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus 30S ribosomal protein S2 (rpsB) CSB-YP532278DTV
CSB-EP532278DTV
CSB-BP532278DTV
CSB-MP532278DTV
CSB-EP532278DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus S-adenosylmethionine synthase (metK) CSB-YP532279DTV
CSB-EP532279DTV
CSB-BP532279DTV
CSB-MP532279DTV
CSB-EP532279DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Hydroxyethylthiazole kinase (thiM) CSB-YP532280DTV
CSB-EP532280DTV
CSB-BP532280DTV
CSB-MP532280DTV
CSB-EP532280DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Enolase (eno) CSB-YP532281DTV
CSB-EP532281DTV
CSB-BP532281DTV
CSB-MP532281DTV
CSB-EP532281DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP532282DTV
CSB-EP532282DTV
CSB-BP532282DTV
CSB-MP532282DTV
CSB-EP532282DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Bifunctional protein GlmU (glmU) CSB-YP532283DTV
CSB-EP532283DTV
CSB-BP532283DTV
CSB-MP532283DTV
CSB-EP532283DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Acetate kinase (ackA) CSB-YP532284DTV
CSB-EP532284DTV
CSB-BP532284DTV
CSB-MP532284DTV
CSB-EP532284DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP532285DTV
CSB-EP532285DTV
CSB-BP532285DTV
CSB-MP532285DTV
CSB-EP532285DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus Dihydroxy-acid dehydratase (ilvD), partial CSB-YP532286DTV
CSB-EP532286DTV
CSB-BP532286DTV
CSB-MP532286DTV
CSB-EP532286DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clavibacter michiganensis subsp. sepedonicus 50S ribosomal protein L1 (rplA) CSB-YP532287DTV
CSB-EP532287DTV
CSB-BP532287DTV
CSB-MP532287DTV
CSB-EP532287DTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Dihydroxy-acid dehydratase (ilvD), partial CSB-YP532288XAZ
CSB-EP532288XAZ
CSB-BP532288XAZ
CSB-MP532288XAZ
CSB-EP532288XAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.pptc.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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